doktorska disertacija
Taja Železnik (Author), Mateja Erdani-Kreft (Mentor), Rok Romih (Thesis defence commission member), Maja Čemažar (Thesis defence commission member), Darja Keše (Thesis defence commission member), Marjanca Starčič Erjavec (Co-mentor)

Abstract

Bolezni sečil zaradi staranja prebivalstva predstavljajo vedno večji izziv. Z namenom iskanja novih terapevtskih pristopov, smo v doktorski disertaciji analizirali učinek pripravkov iz humane amnijske membrane (AM) na rakave urotelijske celice ter uropatogene bakterije. Z različnimi celično-biološkimi, molekularnimi in mikrobiološkimi pristopi smo analizirali učinek nosilcev iz AM, homogenata AM in celic AM na celice T24, ki izvirajo iz humane mišično invazivne urotelijske neoplazije. Ugotovili smo, da 1) nosilci iz AM zavirajo proliferacijo in zmanjšajo invazivni potencial celic T24 ter prispevajo k njihovi mezenhimsko-epitelijski transformaciji, 2) homogenat AM zavira proliferacijo in povzroča luščenje celic T24 ter preprečuje njihovo pritrjanje na podlago in 3) da celice AM zavirajo proliferacijo celic T24. Protimikrobni učinek pripravkov iz AM smo testirali na 25-ih sevih uropatogenih bakterijskih vrst. Dokazali smo, da koščki sveže (svAM) in krioshranjene AM (kAM) nimajo protimikrobnega učinka, medtem ko homogenati svAM in kAM povzročijo cono protimikrobnega učinka pri 16 od 25 testiranih sevov uropatogenih bakterijskih vrst. Protimikrobno delovanje homogenata kAM smo dokazali tudi na z bakterijami okuženih modelih normalnega in rakavo spremenjenega urotelija in vitro. Način priprave ter shranjevanja AM vpliva na obseg njenega protimikrobnega učinka. Ugotovitve, da pripravki iz AM delujejo zaviralno na rakave urotelijske celice in izbrane uropatogene seve uropatogenih bakterijskih vrst, pomembno prispevajo k razumevanju delovanja AM ter predstavljajo izhodišče za nadaljnje študije, ki bodo vrednotile možnosti uporabe AM v urologiji.

Keywords

amnijska membrana;sečni mehur;rakave urotelijske celice;

Data

Language: Slovenian
Year of publishing:
Typology: 2.08 - Doctoral Dissertation
Organization: UL MF - Faculty of Medicine
Publisher: [T. Železnik Ramuta]
UDC: 616-006
COBISS: 34669315 Link will open in a new window
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Other data

Secondary language: English
Secondary title: Analysis of the effect of amniotic membrane-derived preparations in cancer urothelial cells and uropathogenic bacteria
Secondary abstract: The urinary tract diseases pose an increasing challenge due to the aging of population. We aimed to find new therapeutic approaches, thus we analysed the effect of human amniotic membrane-derived (AM) preparations on cancer urothelial cells and uropathogenic bacteria. Using different cell-biological, molecular and microbiological approaches we analysed the effect of AM scaffolds, AM homogenate and AM-derived cells on T24 cells, derived from human muscle-invasive bladder cancer. We demonstrated that 1) AM scaffolds diminish the proliferation and reduce the invasive potential of T24 cells and contribute to their mesenchymal-to-epithelial transformation, 2) AM homogenate diminishes the proliferation and causes detachment of T24 cells and prevents their attachment to the surface and 3) AM-derived cells diminish the proliferation of T24 cells. The antimicrobial effect of AM preparations was tested on 25 strains of uropathogenic bacterial species. We demonstrated that patches of fresh (fAM) and cryopreserved AM (cAM) have no antimicrobial effect, while the fAM and cAM homogenates have antimicrobial effect on 16 out of 25 tested strains of uropathogenic bacterial species. Moreover, cAM homogenate has antimicrobial effect also on bacteria-infected in vitro models of normal and cancerous urothelium. The manner of preparation and storage of AM affects the range of its antimicrobial effect. Findings that AM preparations inhibit cancer urothelial cells and selected strains of uropathogenic bacteria, importantly contribute to to the knowledge of AM activity and serve as a baseline for studies that will evaluate the use of AM in urology.
Secondary keywords: amniotic membrane;bladder;cancer urothelial cells;
Type (COBISS): Dissertation
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. Ljubljana, Medicinska fak.
Pages: XVIII, 126 str., [38] str. pril.
ID: 12058471