diplomsko delo univerzitetnega študijskega programa I. stopnje
Mitja Macur (Author), Maja Leitgeb (Mentor), Katja Heržič (Co-mentor)

Abstract

Namen diplomskega dela je obsegal pripravo imobilizirane hrenove peroksidaze (HRP), in sicer inkapsulirane v obliki alginatnih kroglic, določanje preostale aktivnosti inkapsulirane HRP ter določanje aktivnosti inkapsulirane HRP po skladiščenju pri temperaturah -18 °C, 4 °C, 22 °C in 30 °C. Encim HRP smo inkapsulirali v natrijev alginatni hidrogel z metodo ionotropnega geliranja v raztopini kalcijevega klorida (CaCl2) v obliki sferičnih kroglic. Vzorce kroglic z inkapsulirano HRP smo razdelili na 2 dela ter jih skladiščili v natrijevem acetatnem pufru (pH = 7,8) in na suhem v petrijevki. Rezultate smo primerjali s preostalo aktivnostjo HRP v raztopini in ovrednotili vpliv in način skladiščenja na aktivnost inkapsulirane HRP z izbranim nosilcem. Ugotovili smo nezanemarljiv vpliv alginatnega nosilca na preostalo aktivnost HRP. Rezultati kažejo, da alginatni nosilec učinkovito izboljša aktivnost HRP, medtem ko podaljšanja uporabnosti nismo potrdili. Inkapsulirani HRP v pufru se preostala aktivnost pri temperaturah skladiščenja -18 °C in 4 °C s časom povečuje in je v začetku nižja od preostale aktivnosti HRP, skladiščene pri višji temperaturi. Kot najbolj primeren način se izkaže skladiščenje inkapsulirane HRP v pufru, četudi smo potrdili njeno uhajanje iz alginatnih kroglic.

Keywords

imobilizacija;hrenova peroksidaza;inkapsulacija;natrijev alginat;encimska aktivnost;diplomske naloge;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher: [M. Macur]
UDC: 66.02(043.2)
COBISS: 36418307 Link will open in a new window
Views: 484
Downloads: 75
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Other data

Secondary language: English
Secondary title: Stability of encapsulated horseradish peroxidase
Secondary abstract: Our work comprises the preparation of the immobilized horseradish peroxidase (HRP), encapsulated in the form of alginate beads, determination of residual activity of encapsulated HRP, and determination of activity of encapsulated HRP following its storage at temperatures of -18 °C, 4 °C, 22 °C, and 30 °C. The HRP enzyme was encapsulated in a sodium alginate hydrogel using ionotropic gelation in a calcium chloride solution (CaCl2) in the form of spherical beads. The samples of beads with encapsulated HRP were divided to two parts, and then stored in a sodium acetate buffer (pH = 7,8) or in a dry place in a petri dish. The results were compared to the residual activity of HRP in the solution, after which we evaluated the impact and method of storage on the activity of the encapsulated HRP with the selected carrier. We determined a significant impact of the alginate carrier on the residual activity of HRP. The results show that the alginate carrier efficiently improves the HRP activity, but no extension of usability was confirmed. The residual activity of the encapsulated HRP in buffer at storage temperatures of -18 °C and 4 °C increases over time, and is initially lower than the residual activity of HRP, stored at a higher temperature. Storage of encapsulated HRP in buffer seems to be the most appropriate method, even though we have confirmed enzyme leakage.
Secondary keywords: immobilization;horseradish peroxidase;sodium alginate;enzyme activity;
Type (COBISS): Bachelor thesis/paper
Thesis comment: Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages: 61 f.
ID: 12070816
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