magistrsko delo
Anja Podgrajšek (Author), Jana Ambrožič-Dolinšek (Mentor), Terezija Ciringer (Co-mentor)

Abstract

Arzen je naravno razširjen element, ki lahko toksičen predstavlja nevarnost ljudem, ostalim organizmom in okolju. Z arzenom so onesnažena številna z vodo bogata območja, zato smo želeli raziskati možnosti razstrupljanja tega elementa z makrofitom ozkolistnim koščem (Berula erecta) v tkivni kulturi. Fitoremediacijski potencial B. erecta smo ugotavljali in vitro na Murashige in Scoog (1962) gojišču brez rastnih regulatorjev, ki smo mu po 14 dneh dodali arzenat (0, 0.1, 1, 10, 50 in 100 mg/l). Raziskali smo vpliv arzenata na rastne parametre, fotokemično učinkovitost FS II, vsebnost fotosinteznih barvil, antocianov, vsebnost glutationa in cisteina ter določali privzem arzena v tkivih rastlin. Nizke koncentracije arzena (0.1, 1 in 10 mg/l) so pozitivno vplivale na prirast sveže, suhe mase, na dolžino korenin in poganjkov ter na število brstov in stolonov, zelo očitno predvsem po tretjem tednu poskusa. Visoke koncentracije arzena (50 in 100 mg/l) so zavirale vse rastne parametre in zmanjšale fotokemično učinkovitost. Pri vseh obravnavah z arzenom se je znižala vsebnost fotosintetskih barvil in antocianinov. Privzem celokupnega arzena je naraščal z naraščajočo dodano koncentracijo arzena, intenzivneje v korenine, v primerjavi z nadzemnimi delih rastlin. Vsebnost glutationa in cisteina je v splošnem postopno naraščala z večanjem koncentracije dodanega arzena.

Keywords

magistrska dela;Berula erecta;makrifiti;arzen;tkivne kulture;fitoremediacija;glutation;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UM FNM - Faculty of Natural Sciences and Mathematics
Publisher: [A. Podgrajšek]
UDC: 581.5:582.795(043.2)
COBISS: 48414723 Link will open in a new window
Views: 537
Downloads: 58
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Other data

Secondary language: English
Secondary title: Effect of different arsenate concentrations on growth, development and metabolism of Berula erecta in vitro
Secondary abstract: Arsenic is a naturally widespread element that can be toxic to humans, other organisms and the environment. Many water-rich areas are contaminated with arsenic, so we wanted to investigate the possibilities of detoxifying this element with a macrophyte lesser water-parsnip (Berula erecta) in tissue culture. The phytoremediation potential of B. erecta was determined in vitro on Murashige and Scoog (1962) medium without growth regulators, to which arsenate (0, 0.1, 1, 10, 50 and 100 mg/l) was added after 14 days. Our research should determine the effect of arsenate on growth parameters, the photochemical efficiency of FS II, the content of photosynthetic pigments, anthocyanins, the content of glutathione and cysteine, and the uptake of arsenic in plant tissue. Low concentrations of arsenic (0.1, 1, 10 mg/l) had a positive effect on growth, fresh and dry mass, length of roots and shoots, and number of buds and stolons, which became particularly evident after the third week of the experiment. High concentrations of arsenic (50 and 100 mg/l) inhibited all growth parameters and reduced photochemical efficiency. In all treatments with arsenic, the content of photosynthetic pigments and anthocyanins decreased. The uptake of total arsenic increased with increasing arsenic addition, more in the roots than in the above-ground parts of the plants. The content of glutathione and cysteine generally increased gradually as the concentration of added arsenic increased.
Secondary keywords: master theses;Berula erecta;macrophytes;tissue culture;phytoremediation;glutathione;
Type (COBISS): Master's thesis/paper
Thesis comment: Univ. v Mariboru, Fak. za naravoslovje in matematiko, Oddelek za biologijo
Pages: XIV, 94 str.
ID: 12250668