magistrsko delo
Anja Kos (Author), Tomaž Skrbinšek (Reviewer), Maja Zagmajster (Mentor), Maja Zagmajster (Thesis defence commission member), Tomaž Skrbinšek (Thesis defence commission member), Cene Fišer (Thesis defence commission member), Teo Delić (Thesis defence commission member), Teo Delić (Co-mentor)

Abstract

Namen naloge je z molekularnim pristopom dobiti vpogled v raznolikost strig iz družine Lithobiidae, ki so bile najdene v jamah v Sloveniji, s poudarkom na statusu kompleksa vrst Lithobius stygius. Kompleks vrst L. stygius smo za namen naloge definirali tako, da poleg nominalne vrste vključuje tudi sinonimizirane taksone ter domnevno najbolj sorodno površinsko vrsto, L. schuleri. Najprej smo pridobili zaporedja odsekov za gene COI, 16S rRNA in 28S rRNA za 184 osebkov strig družine Lithobiidae. Izračunali smo filogenetska drevesa po metodi največjega verjetja in Bayesovem pristopu, na podlagi združenih zaporedij in zaporedja za gen COI. Identificirali smo vsaj 12 različnih vrst strig, najdenih v jamah Slovenije. Posebej smo bili pozorni na kompleks L. stygius, kjer smo prepoznali štiri ločene skupine: (i) skupina L. stygius s. str., (ii) najbližji površinski sorodnik skupine L. stygius s. str., to je še neopisana vrsta z območja Snežnika, (iii) skupina še neopisanih jamskih populacij, (iv) Vrsta L. schuleri, ki genetsko ni bližnje sorodna z vrsto L. stygius s. str. S pomočjo delimitacijskih algoritmov ABGD in mPTP smo razločili, da skupino L. stygius s. str. sestavlja štiri ali sedem MOTU-jev (molekularnih operacijskih taksonomskih enot). Vpogled v filogenetske odnose strig najdenih v jamah odpira številna nerešena vprašanja in predstavlja osnovo za nadaljnje raziskave.

Keywords

kriptične vrste;molekularna taksonomija;genetska raznolikost;severni Dinaridi;jame;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [A. Kos]
UDC: 595.62(043.2)
COBISS: 52417539 Link will open in a new window
Views: 372
Downloads: 96
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: English
Secondary title: Genetic variability of the centipede Lithobius stygius species complex in the Northern Dinarides
Secondary abstract: The aim of our research was to investigate the genetic diversity of the centipede family Lithobiidae found in Slovenian caves. The focus of our study was the species complex Lithobius stygius that we defined as the species L. stygius, its synonyms and presumably closest surface relative, L. schuleri. We acquired sequences of genetic markers COI, 16S rRNA and 28S rRNA from 184 specimens. Phylogenetic trees were computed based on COI and joined sequences, with maximum likelihood and the Bayesian method. Results show that at least 12 different centipede species are found in Slovenian caves. The complex L. stygius is, according to phylogenetic analysis, composed of four monophyletic groups: (i) L. stygius s. str., (ii) closest surface relative of the species L. stygius s. str., an undescribed species from mount Snežnik, (iii) an undescribed group of troglomophic populations, (iv) L. schuleri that is not closely related to L. stygius s. str. According to delimitation algorithms ABGD and mPTP, the species L. stygius s. str. is composed of four or seven MOTU (molecular operational taxonomic unit). Insight in phylogenetic relationships of centipedes found in Slovenian caves opens many unsolved questions and as such represents the basis of future researches on centipede diversity.
Secondary keywords: cryptic species;molecular taxonomy;genetic variability;Northern Dinarides;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. Ljubljana, Biotehniška fak.
Pages: XIII f., 49, [21] str.
ID: 12568957