Sinteza s siliko funkcionaliziranih magnetnih nanodelcev za imobilizacijo proteinov

magistrsko delo

Aljaž Degen (Author), Irena Ban (Mentor), Janja Stergar (Co-mentor)

Abstract

Pri tem magistrskem delu smo pripravljali funkcionalizirane magnetitne (Fe3O4) nanodelce, ki bi lahko bili uporabljeni za imobilizacijo encimov tekom procesa čiščenja odpadne vode. Sintetizirane magnetitne nanodelce smo najprej funkcionalizirali s silikatno prevleko (SiO2) in (3-aminopropil) trietoksisilanom (APTES), na koncu je sledila še aktivacija z glutaraldehidom. Za karakterizacijo sintetiziranih magnetitnih nanodelcev in njihovih derivatov smo uporabili več različnih metod. Sestavo magnetitnih nanodelcev smo določali z rentgensko praškovno difrakcijo (RTG); za določitev čistosti pripravljenih nanodelcev in vsebnosti vlage na njihovi površini smo uporabili termogravimetrično analizo (TGA); velikost nanodelcev smo določili s pomočjo rentgenske praškovne difrakcije, z dinamičnim sipanjem svetlobe (DLS) in s transmisijsko elektronsko mikroskopijo (TEM); uspešno funkcionalizacijo derivatov magnetitnih nanodelcev smo določali z meritvami zeta potenciala, infrardečo spektroskopijo s Fourierjevo transformacijo (FTIR) in transmisijsko elektronsko mikroskopijo. Tekom magistrskega dela smo preverjali tudi, ali je potrebno za vezavo APTES-a na magnetitne nanodelce uporabiti silikatno prevleko (posredna vezava), ali lahko dobimo zadovoljive rezultate brez njene uporabe (direktna vezava). Uspeli smo uspešno sintetizirati in funkcionalizirati magnetitne nanodelce ustrezne velikosti s silikatno prevleko in APTES-om. Razlika med vzorcema, ki sta imela direktno in posredno vezan APTES, je minimalna, zato lahko korak funkcionalizacije nanodelcev s silikatno prevleko izpustimo. Uspela je tudi aktivacija nanodelcev z glutaraldehidom, torej so takšni nanodelci pripravljeni za imobilizacijo encimov.

Keywords

Fe3O4 nanodelci;silikatna prevleka;(3-aminopropil) trietoksisilan (APTES);glutaraldehid;imobilizacija encimov;magistrske naloge;

Data

Language:	Slovenian
Year of publishing:	2021
Typology:	2.09 - Master's Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[A. Degen]
UDC:	620.179.141:620.3
COBISS:	66729987
Views:	473
Downloads:	82
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Synthesis of silica functionalized magnetic nanoparticles for protein immobilization
Secondary abstract:	In this master's thesis, we prepared functionalized magnetite (Fe3O4) nanoparticles that could be used for enzyme immobilization during the wastewater treatment process. The synthesized magnetite nanoparticles were first functionalized with a silicate coating (SiO2) and (3-aminopropyl) triethoxysilane (APTES), followed by activation with glutaraldehyde. Several different methods were used to characterize the synthesized magnetite nanoparticles and their derivatives. The composition of magnetite nanoparticles was determined by X-ray powder diffraction (RTG); thermogravimetric analysis (TGA) was used to determine the purity of the prepared nanoparticles and the moisture content on their surface; the nanoparticle size was determined by X-ray powder diffraction, dynamic light scattering (DLS), and transmission electron microscopy (TEM); the successful functionalization of magnetite nanoparticle derivatives was determined by zeta potential measurements, Fourier-transform infrared spectroscopy (FTIR) and transmission electron microscopy. During the master's thesis, we also checked whether it is necessary to use a silicate coating to bind APTES to the magnetite nanoparticles (indirect bonding), or if satisfactory results can be obtained without using it (direct bonding). We were able to successfully synthesize and functionalize magnetite nanoparticles of appropriate size with a silicate coating and APTES. The difference between the samples that had directly and indirectly bound APTES is minimal, so the step of functionalization of nanoparticles with a silicate coating can be omitted. Activation of nanoparticles with glutaraldehyde was also successful, so such nanoparticles are ready to be used for enzyme immobilization.
Secondary keywords:	Fe3O4 nanoparticles;silicate coating;(3-Aminopropyl) triethoxysilane (APTES);glutaraldehyde;enzyme immobilization;
Type (COBISS):	Master's thesis/paper
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	XII, 55 f.
ID:	12795667