magistrsko delo
Špela Klement (Author), Mojca Narat (Reviewer), Darja Keše (Mentor), Barbara Šoba (Co-mentor)

Abstract

Urogenitalne mikoplazme uvrščamo v razred Mollicutes, katerega pripadniki spadajo med najmanjše samodeleče organizme. Nimajo celične stene in so tako edinstveni med prokarionti. Mycoplasma hominis, Ureaplasma parvum, Ureaplasma urealyticum in Mycoplasma genitalium lahko pri ženskah povzročajo vnetje in zaplete v nosečnosti. Virulentni dejavniki jim omogočajo vstop v amnijsko votlino vertikalno po intrauterini poti. Ugotovljeno je, da so urogenitalne mikoplazme najpogostejši mikroorganizmi, izolirani iz amnijske tekočine nosečnic s prezgodnjim porodom, katerih novorojenčki razvijejo bronhopulmonalno displazijo, motnje v razvoju in nevrološka obolenja. Za dokazovanje prisotnosti urogenitalnih mikoplazem najpogosteje uporabljamo metodo verižne reakcije s polimerazo. V magistrski nalogi smo želeli ugotoviti navzočnost urogenitalnih mikoplazem v amnijski tekočini 118 nosečnic. V raziskavo smo vključili 51 vzorcev amnijske tekočine zdravih nosečnic ter 67 vzorcev amnijske tekočine nosečnic, ki so imele patološko nosečnost. Iz amnijske tekočine smo izolirali DNA ter izvedli multiplo verižno reakcijo s polimerazo v realnem času, s čimer smo dokazovali prisotnost DNA bakterij Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis, Mycoplasma genitalium, Chlamydia trachomatis, Neisseria gonorrhoeae in parazita Trichomonas vaginalis. V naši raziskavi nismo dokazali prisotnosti DNA urogenitalnih mikoplazem, ravno tako tudi ne prisotnosti C. trachomatis, N. gonorrhoeae in T. vaginalis v amnijski tekočini tako zdravih, kot tudi nosečnic s patološko nosečnostjo.

Keywords

urogenitalne mikoplazme;Mycoplasma hominis;Ureaplasma spp.;nosečnice;amnijska tekočina;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL MF - Faculty of Medicine
Publisher: [Š. Klement]
UDC: 579.887:616.6-055.26
COBISS: 61745411 Link will open in a new window
Views: 465
Downloads: 96
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Other data

Secondary language: English
Secondary title: Detection of urogenital mycoplasmas in amniotic fluid in pregnant women
Secondary abstract: Urogenital mycoplasmas belong to the class Mollicutes and are known to be the smallest self-growing organisms. They have no cell wall and are therefore unique among prokaryotes. Mycoplasma hominis, Ureaplasma parvum, Ureaplasma urealyticum, and Mycoplasma genitalium may cause infections and complications during pregnancy. Virulence factors enable them to enter the amniotic cavity vertically through the intrauterine route. Urogenital mycoplasmas have been found to be the most common microorganisms isolated from the amniotic fluid of women with preterm birth, whose newborns develop bronchopulmonary dysplasia, developmental disorders, and neurological diseases. The polymerase chain reaction (PCR) is the most commonly-used method for detection of urogenital mycoplasmas. In our master's thesis, we wanted to determine the presence of urogenital mycoplasmas in the amniotic fluid of 118 pregnant women. The study included 51 DNA isolates from the amniotic fluid of healthy pregnant women and 67 samples of the amniotic fluid from pregnant women who had a pathological pregnancy. We isolated the DNA from the amniotic fluid and performed a real-time polymerase chain reaction, whereby we proved the presence of DNA of Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis, Mycoplasma genitalium, Chlamydia trachomatis, Neisseria gonorrhoeae, and the parasite Trichomonas vaginalis. In our study, we did not demonstrate the presence of urogenital mycoplasmas in the amniotic fluid nor the presence of C. trachomatis, N. gonorrhoeae, or T. vaginalis of both healthy pregnant women and pregnant women with pathological pregnancies.
Secondary keywords: urogenital mycoplasmas;Mycoplasma hominis;Ureaplasma spp.;pregnant women;amniotic fluid;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij mikrobiologije
Pages: X, 59 f.
ID: 12839667