magistrsko delo
Leon Ostanek Jurina (Author), Polona Žnidaršič Plazl (Mentor), Aleš Podgornik (Thesis defence commission member), Igor Plazl (Thesis defence commission member)

Abstract

Intenzifikacija procesov na osnovi miniaturizacije, visoka zmogljivost, nizki stroški obratovanja in potrošnega materiala, laminarni tokovni režimi, precizen nadzor in možnost manipulacije toka tekočin, učinkovit prenos toplote in snovi. Vse to in še več so lastnosti, ki definirajo mikrofluidiko, vedo, ki je spremenila paradigmo biokemijskih in kemijskih raziskav ter načrtovanja procesov v zadnjih treh desetletjih. Ena izmed podskupin mikrofluidike je kapljična mikrofluidika, ki deluje na principu ustvarjanja kapljic s prostorninami med 10-9 L in 10-15 L, ki jih lahko uporabimo za pripravo mikrobioreaktorjev za karakterizacijo posameznih celic. Glavna uporabnost takšnih sistemov je izvedba hitrih presejalnih testov, s katerimi povišamo pretočnost eksperimentov za faktor več tisoč in hkrati občutno znižamo stroške njihove izvedbe. Glavni kriteriji za uspešnost takšnega sistema so pravilen izbor posameznih komponent dvofaznega sistema, stabilnost nastale emulzije, uspešna inkapsulacija biološkega materiala v kapljice ter njihova detekcija in sortiranje glede na analizo vsebine. V nalogi je predstavljen razvoj mikropretočne platforme za izvedbo hitrih presejalnih testov na osnovi detekcije vsebine kapljic in nadaljnjega sortiranja. Opisane so različne komponente in pogoji tvorbe kapljic, ki zagotavljajo tvorbo stabilne in monodisperzne emulzije na različnih mikrofluidnih čipih. Opisan je postopek izdelave mikrofluidnega čipa iz polidimetilsiloksana za pripravo kapljic, ki v nadaljevanju omogoča tudi povezavo z ustreznim detektorjem riboflavina in sortiranje kapljic. V okviru predstavljenega dela sem uspel razviti detekcijo vsebine kapljic, ki so vsebovale različne koncentracije standarda riboflavina s sklopljenim sistemom čipa in detektorja absorbance. Razviti sistem bo v nadaljevanju omogočal visokozmogljivostni presejalni test za izbor ustreznega produkcijskega mikroorganizma za proizvodnjo riboflavina.

Keywords

kapljična mikrofluidika;polidimetilsioksan;mikrobioreaktorji;mikrofluidne napreve;mikrofluidni čipi;visokozmogljivostni presejalni testi;detekcija vsebine kapljic;detekcija mikrobne aktivnosti;magistrska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [L. Ostanek Jurina]
UDC: 66.02:602(043.2)
COBISS: 65845763 Link will open in a new window
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Downloads: 55
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Other data

Secondary language: English
Secondary title: Development of a microfluidic platform for microdroplet generation and analysis
Secondary abstract: Process intensification by miniaturization, high-throughput, low operating and material costs, laminar fluid flow, precise control, and fluid manipulation, efficient heat and mass transfer. All of the above and more are key properties that define microfluidics, a science that has changed the paradigm of biochemical and chemical research and process development in the last three decades. Droplet-based microfluidics is a subclass of microfluidics, where small droplets from 10-9 L to 10-15 L are generated. Droplets serve as isolated microbioreactors where various biological experiments can be performed. High throughput screening (HTS) experiments are the main and most important use of droplet-based microfluidics. A 1000-fold increase in throughput and a significant reduction of assay reagent cost can be obtained by using microfluidics for screening experiments. The main criteria for successful usage of a droplet-based microfluidics platform are an adequate selection of chemicals of a two-phase system, emulsion stability, successful encapsulation of biological material in droplets, detection and sorting, based on content analysis. Development of a droplet microfluidic platform with intent to use it for HTS experiments is presented. Chemicals that meet the criteria for use in droplet microfluidics are reported. Different droplet generation conditions which ensure the creation of stable and monodisperse emulsion on various microfluidic chips are presented. The process of droplet generation chip made from polydimethylsiloxane fabrication is described. The coupled system of microfluidic chip and optical detector ODcube instant proved to be successful in carrying out detection experiments on droplets based on different concentrations of riboflavin standard laden droplets and has the potential to be used in future in a HTS experiment for selection of the best riboflavin producing microorganism mutant.
Secondary keywords: droplet microfluidics;polydimethylsiloxane;microbioreactors;high-throughput screening;droplet detection;
Type (COBISS): Master's thesis/paper
Study programme: 1000376
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, smer Kemijsko inženirstvo
Pages: XIII f., 73 str.
ID: 12891443