diplomsko delo
Ana Rupar (Author), Nataša Štajner (Mentor)

Abstract

Na mikroalgah osnovane biotehnološke platforme zaenkrat še niso enakovredne ostalim platformam, ki se uporabljajo v biotehnologiji (bakterije, kvasovke, rastlinske in živalske celične kulture, rastline itd.). Za svojo rast v težkih pogojih proizvajajo razne metabolite, ki so zanimivi tudi za prehrambno industrijo in zdravstvo. Z vidika transformacije pa so zanimivi predvsem kloroplasti, ki so značilni za rastlinske celice in cianobakterije ter imajo velik biotehnološki potencial. Zaradi svojega razvoja, iz prej samostojnih prokariontskih organizmov, je njihov genom (plastom) manj kompleksen in ga lažje modificiramo. Ta metoda transformacije plastoma se uporablja za pridobivanje širokega spektra rekombinantnih proteinov in tudi drugih spojin. S homologno rekombinacijo se v plastom lahko vstavi gene sorodnih mikroalg ali drugih organizmov. Vstavi se lahko enega ali več genov, spreminja metabolne poti, ki so že prisotne v kloroplastih. V diplomskem delu so predstavljene tudi možne slabosti transformacije kloroplastov ter predlogi za izboljšave. Na dveh primerih je prikazana uspešna transformacija in produkcija industrijsko pomembnih fitaze in astaksantina.

Keywords

mikroalge;plastom;Chlamydomonas reinhardtii;industrijski proizvodi;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [A. Rupar]
UDC: 606:602.3:579.871:602.6:582.303/.305:604.4(043.2)
COBISS: 68240387 Link will open in a new window
Views: 249
Downloads: 25
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Other data

Secondary language: English
Secondary title: Transformation of microalgal chloroplasts to obtain valuable industrial products
Secondary abstract: Microalgae-based biotechnology platforms are not yet equivalent to other platforms used in biotechnology (bacteria, yeast, plant and animal cell cultures, plants etc.). For their growth in harsh environments, they produce various metabolites that are interesting for food industry as well as healthcare. Chloroplasts, which are specific for plant cells and cyanobacteria, have great biotechnological potential and are particularly interesting for transformation. Because of their evolution from previously autonomous prokaryotic organism, their genome (plastome) is less complex and easier to modify. This method of plastome transformation is used to obtain a wide specter of recombinant proteins and other compounds. By homologous recombination, genes of related microalgae or other organisms can be inserted into the plastome. One or more genes can be inserted, in addition the already present metabolic pathways can be modified. The diploma thesis also presents possible disadvantages of chloroplast transformation and suggestions for improvements. Two cases are shown to present successful transformation and production of industrially important phytase and astaxantin.
Secondary keywords: microalgae;industrial products;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: VI, 20 str.
ID: 13069929