magistrsko delo
Martin Kregar (Author), Matej Kranjc (Mentor)

Abstract

Elektroporacija je pojav, pri katerem pride do povečanja prepustnosti celične membrane zaradi izpostavitve celice električnemu polju. Elektroporacija je bila in je še vedno predmet intenzivnih raziskav na številnih področjih, kot so biomedicina, v predelavi hrane in kemijskem inženirstvu, prav tako pa tudi v okolijskih znanostih. Pri veliko študijah se uporablja za preučevanje porazdelitve električnega polja pri elektroporaciji surov gomolj krompirja, saj območje, ki je izpostavljeno elektroporaciji, zaradi oksidacije fenolnih spojin potemni že v roku 5 ur po dovajanju elektroporacijskih pulzov. Cilj magistrskega dela je preučevanje elektroporacije na gomolju krompirja in določitev pragovne vrednosti električnega polja za dosego ireverzibilne elektroporacije. Za ta namen sem izvedel poskuse na različnih sortah gomolja krompirja pri različnih amplitudah dovedenih pulzov, meritev impedance pred in po elektroporaciji in meritev napetosti ter toka dovedenih električnih pulzov. Analiza elektroporiranega dela gomolja krompirja je temeljila na zajemu slik gomolja in segmentaciji po izvedeni elektroporaciji. Za ovrednotenje in določevanje praga ireverzibilne elektroporacije sem izdelal numerični model gomolja krompirja in s pomočjo metode končnih elementov izračunal porazdelitev električnega polja, ki se vzpostavi med elektroporacijo. S primerjavo površine numeričnega modela in segmentiranega dela gomolja sem določil pragovno vrednost električnega polja, za doseganje ireverzibilne elektroporacije na gomolju krompirja.

Keywords

elektroporacija;gomolj krompirja;metoda končnih elementov;električno polje;segmentacija;magisteriji;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL FE - Faculty of Electrical Engineering
Publisher: [M. Kregar]
UDC: 602.621:582.930.11(043.2)
COBISS: 68691715 Link will open in a new window
Views: 364
Downloads: 78
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Other data

Secondary language: English
Secondary title: Study of electroporation on potato tuber
Secondary abstract: Electroporation is a phenomenon where is an increase in permeability of the cell membrane due to the exposure of the cell to an electric field. Electroporation has been and continues to be the subject of intensive research in many fields, such as biomedicine, food processing and chemical engineering, as well as in environmental sciences. In many studies, a raw potato tuber is used to study electric field distribution established during electroporation, as exposed area darkens due to oxidation of phenolic compounds within 5 hours after electroporation pulses. The aim of thesis is to study electroporation on potato tuber and determine the threshold of electric field to achieve irreversible electroporation. For this purpose, we performed experiments on different varieties of potato tubers at different delivered pulses amplitudes, measuring the impedance before and after electroporation and measuring the voltage and current of the supplied electric pulses. The analysis of the electroporated part of the potato tuber was based on the captured images of the potato tuber and segmentation after electroporation. We made a numerical model of the potato tuber using finite element method to evaluate and determine threshold of irreversible electroporation and calculated distribution of the electric field during electroporation. The threshold value of the electric field for irreversible electroporation was determined by comparing area of the numerical model and segmented part of the potato tuber.
Secondary keywords: electroporation;potato tuber;finite element method;electric field;segmentation;
Type (COBISS): Master's thesis/paper
Study programme: 1000316
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za elektrotehniko
Pages: 67 str.
ID: 13085155