Določevanje N-glikanov z masno spektrometrijo

diplomsko delo

Veronika Godec (Author), Matevž Pompe (Mentor)

Abstract

Pred masnospektrometrično analizo je N-glikane potrebno sprostiti iz glikoproteinov, nato pogosto sledi derivatizacija N-glikanov za izboljšanje občutljivosti masne spektrometrije, učinkovitejšo ionizacijo spojin in zaščito funkcionalnih skupin. Ker se z masno spektrometrijo ne da razlikovati med vsemi izomeri, se te loči s sklopitvijo s separacijsko tehniko. Najpogosteje se za ionizacijo uporabljata laserska desorpcija s pomočjo matrice in elektrorazprševanje, ki povzročita malo ali nič fragmentacije. Iz masnega spektra se tako lahko določi molekulska masa analita. Informacijo o strukturi N glikana se dobi iz tandemskega masnega spektra na podlagi ionskih fragmentov. Z visokoločljivostno masno spektrometrijo je možno razlikovati med izobarnimi spojinami. N-glikane se lahko detektira tudi z drugimi tehnikami, vendar le masna spektrometrija poda točno molekulsko maso in informacijo o strukturi analita.

Keywords

analiza N-glikanov;derivatizacija N-glikanov;masna spektrometrija;ionizacija;laserska desorpcija s pomočjo matrice;MALDI-MS;elektrorazprševanje;ESI-MS;diplomska dela;

Data

Language:	Slovenian
Year of publishing:	2021
Typology:	2.11 - Undergraduate Thesis
Organization:	UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher:	[V. Godec]
UDC:	543.51(043.2)
COBISS:	71704067
Views:	325
Downloads:	45
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Determination of N-glycans by mass spectrometry
Secondary abstract:	Before mass spectrometry analysis, N-glycans must be released from glycoproteins, usually followed by N-glycan derivatization. Derivatization improves sensitivity in mass spectrometry, ionization efficiency and protects functional groups. A lot of isomers cannot be distinguished by mass spectrometry analysis, therefore coupling with separation technique is needed to separate isomers. Matrix-assisted laser desorption/ionization and electrospray ionization are most common ionization modes, which cause little fragmentation or none at all. Molecular mass of analyte can be determined from mass spectrum. N-glycan structural information can be provided by ion fragments in tandem mass spectrum. Isobaric compounds can be differentiated by high-resolution mass spectrometry. N-glycans can be detected by other detection techniques as well, but accurate molecular mass and structural information can be provided by mass spectrometry only.
Secondary keywords:	N-glycan analysis;N-glycan derivatization;mass spectrometry;matrix-assisted laser desorption;electrospray ionization;
Type (COBISS):	Bachelor thesis/paper
Study programme:	1000373
Embargo end date (OpenAIRE):	1970-01-01
Thesis comment:	Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, UNI Kemija
Pages:	30 str.
ID:	13112530