diplomsko delo
Sergeja Šetina (Author), Jernej Ogorevc (Mentor)

Abstract

Za zdravljenje bolezni je ključno razumevanje procesov, ki vodijo v njihov nastanek. Preučevanje molekularnih mehanizmov bolezni pogosto poteka na celičnih linijah. Z reprogramiranjem somatskih celic lahko vzpostavimo linije induciranih pluripotentnih matičnih celic, ki izvirajo iz pacientov s kandidatno mutacijo za določeno bolezen. Ker različna genetska ozadja pacientov pogosto otežujejo primerjavo celičnih linij, lahko s pomočjo orodij za preurejanje genoma vzpostavimo izogene celične linije, ki se med seboj razlikujejo le v kandidatni mutaciji. Zaradi različnih genotipov, imajo lahko zdravila na bolnike različne učinke, zato so celične linije, vzpostavljene iz tkiva pacientov, uporabne tudi za testiranje zdravil na bolniku lastnih celicah, s čimer lahko ugotovimo, katero zdravilo je za posameznike najbolj primerno.

Keywords

biotehnologija;celični modeli;genski inženiring;precizna medicina;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [S. Šetina]
UDC: 602.69:602.9:611.018:606:61(043.2)
COBISS: 70054147 Link will open in a new window
Views: 347
Downloads: 72
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Other data

Secondary language: English
Secondary title: Genetically edited cell lines for disease modelling
Secondary abstract: Understanding molecular processes, causing diseases, is crucial for treatment development. Various cell lines have often been used to study disease mechanisms. By reprogramming the somatic cells, we can establish induced pluripotent stem cell lines, which contain patients' candidate mutation(s) for the specific disease. Different genetic backgrounds of patients often interfere with understanding specific genotype-fenotype relationship. Therefore, it is often helpful, by the use of genome editing technology, to establish isogenic cell lines that differ only in candidate mutation. Due to the different genotypes of patients, drugs often show individual-specific effects. Drug testing on patient-specific cell lines enables selection of drugs which are the most appropriate for a particular patient.
Secondary keywords: biotechnology;cellular models;genetic engineering;precision medicine;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: VI, 22 str.
ID: 13137064