Eva Lasič (Author),
Marjeta Lisjak (Author),
Anemari Horvat (Author),
Mićo Božić (Author),
Aleksandra Šakanović (Author),
Gregor Anderluh (Author),
Alexei Verkhratsky (Author),
Nina Vardjan (Author),
Jernej Jorgačevski (Author),
Matjaž Stenovec (Author),
Robert Zorec (Author)
Abstract
Ketamine is an antidepressant with rapid therapeutic onset and long-lasting effect, although the underlying mechanism(s) remain unknown. Using FRET-based nanosensors we found that ketamine increases [cAMP]i in astrocytes. Membrane capacitance recordings, however, reveal fundamentally distinct mechanisms of effects of ketamine and [cAMP]i on vesicular secretion: a rise in [cAMP]i facilitated, whereas ketamine inhibited exocytosis. By directly monitoring cholesterol-rich membrane domains with a fluorescently tagged cholesterol-specific membrane binding domain (D4) of toxin perfringolysin O, we demonstrated that ketamine induced cholesterol redistribution in the plasmalemma in astrocytes, but neither in fibroblasts nor in PC 12 cells. This novel mechanism posits that ketamine affects density and distribution of cholesterol in the astrocytic plasmalemma, consequently modulating a host of processes that may contribute to ketamine's rapid antidepressant action.
Keywords
astrocytes;ketamine;cholesterol;
Data
Language: |
English |
Year of publishing: |
2019 |
Typology: |
1.01 - Original Scientific Article |
Organization: |
UL MF - Faculty of Medicine |
UDC: |
616-092 |
COBISS: |
34434009
|
ISSN: |
2045-2322 |
Views: |
294 |
Downloads: |
74 |
Average score: |
0 (0 votes) |
Metadata: |
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Other data
Secondary language: |
Slovenian |
Secondary keywords: |
astrociti;ketamin;holesterol; |
Type (COBISS): |
Article |
Pages: |
str. 1-12 |
Issue: |
ǂVol. ǂ9 |
Chronology: |
July 2019 |
DOI: |
10.1038/s41598-019-47459-z |
ID: |
13181174 |