diplomsko delo
Tilen Pajk (Author), Jernej Jakše (Mentor)

Abstract

Nanopore sekvenciranje je perspektivna nova tehnologija z začetki uporabe v prejšnjem tisočletju. Deluje na podlagi zaznavanja sprememb v toku, ki teče skozi poro v membrani. Spremembe toka povzroča prehajanje molekule skozi nanoporo, ki je vezana v membrano. Oxford Nanopore Technologies je podjetje, ki je odgovorno za mnogo napredkov na tem področju in je leta 2014 izdalo napravo MinION, ki ponuja mnoge prednosti pred ostalimi metodami sekvenciranja nove generacije. Čeprav so imele začetne različice težave z doseganjem visoke natančnosti, so izboljšave naprave to dvignile do standardov, ki so postavljene pri ostalih tehnologijah nove generacije. Zahteve moderne diagnostike se pomikajo stran od tradicionalnih metod proti hitrejšim tehnologijam, ki lahko rezultate podajo v krajšem ali celo realnem času. Nanopore tehnologija se je dokazala kot nepogrešljiva pri spremljanju in ukrepanju v mnogih izbruhih ebole in drugih tropskih bolezni, kjer so fizične lastnosti in zmogljivosti MinION-a ekipam omogočile sekvenciranje na prej nedostopnih lokacijah in s tem izboljšale nadzor situacije ter omogočile natančnejši vpogled v spreminjanje patogenov skozi potek izbruhov. Hitrost sekvenciranja, zmogljivost podajanja rezultatov v realnem času, lahka uporaba in prenosljivost, so razlogi, da se MinION naprava vedno več uporablja v diagnostične namene za živali, rastline in druge vrste povsod po svetu.

Keywords

nanopore;MinION;diagnostika;sekvenciranje;bioinformatika;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [T. Pajk]
UDC: 601.4:577.21:575.112(043.2)
COBISS: 75418115 Link will open in a new window
Views: 286
Downloads: 40
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Other data

Secondary language: English
Secondary title: Bioinformatic analysis in diagnostics using nanopore technology
Secondary abstract: Nanopore sequencing is a promising new technology with beginnings in the previous millennium. It works by detecting changes in the electrical current flowing through a nanopore embedded in the membrane. The changes are caused by the passage of a charged molecule through a nanopore bound to the membrane. Oxford Nanopore Technologies is the company responsible for many advances in this field. In 2014, they released the MinION device, which offers many advantages over other next-generation sequencing methods. Although the first versions struggled to achieve high accuracy, improvements have raised the device to the standard of other next-generation technologies. The demands of modern diagnostics are moving away from traditional methods to faster technologies that can deliver results faster or even in real time. Nanopore technology has proven essential in monitoring and responding to many outbreaks of Ebola and other tropical diseases. The physical properties and capabilities of the MinION have allowed teams to sequence in previously inaccessible locations, improving situational control and providing more accurate insights into how pathogens change over the course of outbreaks. Sequencing speed, real-time result delivery, ease of use and portability are the reasons why the MinION instrument is increasingly used for diagnostic purposes in animals, plants and other species around the world.
Secondary keywords: diagnostics;sequencing;bioinformatics;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: VI, 19 str.
ID: 13292548