Active protein aggregates produced in Escherichia coli

Špela Peternel (Author), Radovan Komel (Author)

Abstract

Since recombinant proteins are widely used in industry and in research, the need for their low-cost production is increasing. Escherichia coli is one of the best known and most often used host organisms for economical protein production. However, upon over-expression, protein aggregates called inclusionbodies (IBs) are often formed. Until recently IBs formation represented a bottleneck in protein production as they were considered as deposits of inactive proteins. However, recent studies show that by choosing the appropriate host strain and designing an optimal production process, IBs composed from properly folded and biologically active recombinant proteins canbe prepared. Such active protein particles can be further used for the isolation of pure proteins or as whole active protein particles in various biomedical and other applications. Therefore interest in understanding the mechanisms of their formation as well as their properties is increasing.

Keywords

E. coli;active protein aggregates;inclusion bodies;IBs;recombinant proteins;biotechnology;

Data

Language:	English
Year of publishing:	2011
Typology:	1.02 - Review Article
Organization:	UL MF - Faculty of Medicine
UDC:	577
COBISS:	29115609
ISSN:	1422-0067
Views:	286
Downloads:	100
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	Escherichia Coli;Metabolism;Recombinant Proteins;Biosynthesis;Inclusion Bodies;Biotechnology;Escherichia coli;Rekombinantne beljakovine;Inkluzijska telesca;Biotehnologija;
Type (COBISS):	Article
Pages:	str. 8275-8287
Issue:	ǂVol. ǂ12
Chronology:	2011
DOI:	10.3390/ijms12118275
ID:	13324743