diplomsko delo
Gregor Puhar (Author), Matevž Pompe (Mentor)

Abstract

Desnosučne aminokisline so v majhnih količinah prisotne tako v sintetičnih kot tudi v nekaterih naravnih peptidih in proteinih. Določevanje vezavnih mest desnosučnih aminokislin je pomembno za podrobno analizo in razumevanje teh peptidov in proteinov. V diplomskem delu sem predstavil metode za analizo proteinov, ki vsebujejo desnosučne aminokisline. Vse metode za določevanje vezavnih mest desnosučnih aminokislin temeljijo na masni spektrometriji, ki je ključna tehnika za analizo zgradbe proteinov. Cilj eksperimentalnega dela je bil razviti metodo za ločbo proteinov, ki vsebujejo desnosučne aminokisline. Metodo sem razvijal na dveh tandemskih masnih spektrometrih, ki sta bila sklopljena s tekočinskima kromatografoma.

Keywords

D-aminokisline;kiralnost;stereoizomeri;peptidi;proteini;masna spektrometrija;proteomika;diplomska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [G. Puhar]
UDC: 543.51:547.1-32-304.2(043.2)
COBISS: 79533059 Link will open in a new window
Views: 253
Downloads: 29
Average score: 0 (0 votes)
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Other data

Secondary language: English
Secondary title: Determination of bonding sites of D-amino acids in peptides and proteins
Secondary abstract: D-amino acids are found in small quantities both in natural and synthetic peptides and proteins. Determination of their bonding sites is important for detailed analysis and understanding of these peptides and proteins. In my diploma thesis I presented methods for analysis of proteins that consist of D-amino acids. All methods for determination of bonding sites of D-amino acids are based on mass spectrometry, which is the key technique for analysing the structure of proteins. The goal of the experimental part was to develop a method to separate proteins consisting of D-amino acids. The method was developed with two tandem mass spectrometers, which were coupled with liquid chromatography instruments.
Secondary keywords: D-amino acids;peptides;proteins;stereoisomers;mass spectrometry;
Type (COBISS): Bachelor thesis/paper
Study programme: 1000373
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, UNI Kemija
Pages: 30 str.
ID: 13331916