diplomsko delo
Špela Rupnik (Author), Katarina Vogel-Mikuš (Mentor)

Abstract

Plazma predstavlja eno od štirih osnovnih agregatnih stanj snovi in jo lahko delimo na visokotemperaturno in nizkotemperaturno oziroma toplotno in hladno. V kmetijstvu se uporablja hladno plazmo za obdelavo semen, ker se ob primernih nastavitvah plazemskih parametrov kot so npr. vrsta plina, moč in dolžina izpostavitve, lahko izboljša hitrost kalitve, rast sadik, omočljivost semen in poveča donos pridelka. Večja omočljivost je posledica jedkanja semenske ovojnice, s čimer se poveča njena hidrofilnost. Poleg tega lahko vpliva še na gensko ekspresijo, sintezo hormonov in odpornost na biotski (patogeni) in abiotski stres (suša, slanost, težke kovine). Spremeni se predvsem izražanje genov, ki kodirajo rastlinske hormone in s tem pride do spremenjenega ravnovesja rastlinskih hormonov. Poveča se namreč izražanje giberelinske kisline, zmanjša pa izražanje abscizinske kisline, kar pripomore tudi k premagovanju dormance semen. Prav tako lahko pride do inaktivacije patogenov (tako bakterij, gliv in tudi virusov) in povečanega izražanja rezistentnih genov v rastlinah (geni PR). Zato bi lahko hladna plazma v prihodnosti nadomestila kemična sredstva namenjena kontroli rastlinskih škodljivcev. Večino sprememb lahko pripišemo delovanju reaktivnih atomskih in molekularnih zvrsti kot so ROS in RNS, ki so sestavni del hladne plazme.

Keywords

seme;obdelava;hladna plazma;kalitev;izražanje genov;dekontaminacija;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [Š. Rupnik]
UDC: 631.53.027:601.2:576.31:581.17(043.2)
COBISS: 76604419 Link will open in a new window
Views: 212
Downloads: 21
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Other data

Secondary language: English
Secondary title: Use of non-thermal plasma technology for seed treatment
Secondary abstract: Plasma represents one out of the four fundamental states of matter and can be distinguished into thermal and non-thermal or cold plasma. In agriculture, cold plasma is used for seed treatment, as it can improve germination rate, seedling growth, seed wettability and increase crop yield with appropriate settings of plasma parameters, such as type of gas, strength and length of exposure. The higher wettability is due to the etching of the seed coat, thus increasing its hydrophilicity. In addition, it can affect gene expression, hormone synthesis, and resistance to biotic (pathogen) and abiotic stress (drought, salinity, heavy metals). In particular, the expression of genes encoding plant hormones changes, leading to an altered balance of plant hormones. Namely, the expression of gibberellic acid increases and the expression of abscisic acid decreases which also helps to overcome seed dormancy. Inactivation of pathogens (both bacteria, fungi and also viruses) and increased expression of resistant genes in plants (PR genes) can also occur. For this reason, cold plasma could in the future replace chemical agents intended for plant pest control. Most of the changes can be attributed to the action of reactive atomic and molecular species such as ROS and RNS which are an integral part of cold plasma.
Secondary keywords: seed;treatment;cold plasma;germination;gene expression;decontamination;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: VIII, 20 str.
ID: 13381049