Urška Dragin (Author), Marko Kolenc (Author), Andrej Steyer (Author), Peter Veranič (Author), Mateja Poljšak-Prijatelj (Author), Mateja Erdani-Kreft (Author)

Abstract

Contamination of cell cultures is the most common problem encountered in cell culture laboratories. Besides the secondary cell contaminations often occurring in the cell laboratories, the contaminations originating from donor animal or human tissue are equally as common, but usually harder to recognize and as such require special attention. The present study describes the detection of porcine adenovirus (PAdV), strain PAdV-SVN1 in cultures of normal porcine urothelial (NPU) cells isolated from urinary bladders of domestic pigs. NPU cell cultures were evaluated by light microscopy (LM), polymerase chain reaction (PCR), and additionally assessed by transmission electron microscopy (TEM). Characteristic ultrastructure of virions revealed the infection with adenovirus. The adenoviral contamination was further identified by the sequence analysis, which showed the highest similarity to recently described PAdV strain PAdV-WI. Additionally, the cell ultrastructural analysis confirmed the life-cycle characteristic for adenoviruses. To closely mimic the in vivo situation, the majority of research on in vitro models uses cell cultures isolated from human or animal tissue and their subsequent passages. Since the donor tissue could be a potential source of contamination, the microbiological screening of the excised tissue and harvested cell cultures is highly recommended.

Keywords

porcine adenovirus;cell culture;urinary bladder;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL MF - Faculty of Medicine
UDC: 577
COBISS: 31479257 Link will open in a new window
ISSN: 1999-4915
Views: 200
Downloads: 58
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Other data

Secondary language: Slovenian
Secondary keywords: prašičji adenovirus;celične kulture;sečni mehur;
Type (COBISS): Article
Pages: str. 2505-2518
Issue: ǂVol. ǂ6
Chronology: Jun. 2014
DOI: 10.3390/v6062505
ID: 13469977