magistrsko delo
Edina Iljazović (Author), Elvira Maličev (Mentor), Anže Abram (Co-mentor)

Abstract

Mezenhimske matične celice predstavljajo heterogeno skupino celic, ki jih lahko izoliramo iz številnih tkiv odraslega človeka. Zanimive so predvsem zaradi njihove sposobnosti migracije na mesto vnetja v telesu, kjer izločajo različne topne dejavnike kot so citokini, kemokini in rastni dejavniki, ki izboljšujejo angiogenezo, zavirajo vnetje in inhibirajo apoptozo. Zaradi številnih imunomodulatornih in regenerativnih lastnosti je vedno več zanimanja za uporabo mezenhimskih matičnih celic v kliniki pri številnih patoloških stanjih, za katere sta značilna vnetje in degeneracija tkiva. Vse večji obseg uporabe zahteva tudi vse boljše tehnike ločevanja celic, ki morajo biti hitrejše in bolj učinkovite. Namen naloge je bil raziskati potencialne materiale, ki bi bili ustrezni za ločevanje mezenhimskih matičnih celic od ostalih celic. V raziskovalni nalogi smo uporabili humane mezenhimske matične celice, izolirane iz kostnega mozga. Najprej smo okarakterizirali vzorce materialov z izbranimi tehnikami, in sicer smo merili zeta potencial, kontaktni kot ter hrapavost. V nadaljevanju smo ugotavljali vpliv lastnosti materialov na obnašanje celic – na njihovo adhezijo. Za najbolj učinkovit material se je izkazala mikrotitrska plošča s fibronektinom, na katero se je vezalo največ mezenhimskih matičnih celic. Na ostalih materialih nismo dobili ponovljivih rezultatov, zato nismo prišli do jasnih zaključkov. Ugotovili smo, da so interakcije med celicami in materiali zelo kompleksne in da je za njihovo razumevanje potrebno še precej raziskav. Iskanje novih metod ločevanja celic je težavno, saj je nemogoče določiti enostaven kriterij za uspešno adhezijo in nato ločevanje celic.

Keywords

mezenhimske matične celice;ločevanje celic;adhezija;polimeri;zeta potencial;mejni kot;hrapavost;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [E. Iljazović]
UDC: 602.9:611.018(043.2)
COBISS: 79025667 Link will open in a new window
Views: 5059
Downloads: 39
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Other data

Secondary language: English
Secondary title: Determining the suitability of polymeric materials for mesenchymal stem cell separation
Secondary abstract: Mesenchymal stem cells represent a heterogeneous group of cells that can be isolated from several adult tissues. In recent years the main interest is in their ability to migrate to the site of inflammation in the body, where they secrete various soluble factors such as cytokines, chemokines, and growth factors that improve angiogenesis, inhibit inflammation, and inhibit apoptosis. Their immunomodulatory and regenerative properties are the reason for increasing interest for clinical applications, where they could be used for treatment of many pathological conditions related to inflammation and tissue degeneration. Increasing use requires better cell separation techniques, which need to be faster and more efficient. The purpose of our research was to investigate potential materials which would be suitable for mesenchymal stem cell separation from other cells. We used bone marrow derived human mesenchymal stem. In the first part of the research, we characterized sample materials via measuring the zeta potential, contact angle and roughness. In the second part, we determined the influence of the material properties on the behavior of cells - on their adhesion. The best results were shown on the fibronectin coated microtiter plate, as the largest proportion of mesenchymal stem cells adhered to this material. We did not get consistent results on the other materials, therefore we did not come to clear conclusions. We have confirmed that cell-material interactions are very complex and still need to be researched in detail. Finding new methods for cell separation is difficult, as it is impossible to determine simple criteria for successful adhesion and following cell separation.
Secondary keywords: mesenchymal stem cells;cell separation;adhesion;polymers;zeta potential;contact angle;surface roughness;
Type (COBISS): Master's thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: XVI, 75 f.
ID: 13620754