Ajay Kumar Mishra (Author), Atul Kumar (Author), Deepti Mishra (Author), Vishnu Sukumari Nath (Author), Jernej Jakše (Author), Tomáš Kocábek (Author), Uday Kumar Killi (Author), Filis Morina (Author), Jaroslav Matoušek (Author)

Abstract

Viroids are smallest known pathogen that consist of non-capsidated, single-stranded non-coding RNA replicons and they exploits host factors for their replication and propagation. The severe stunting disease caused by Citrus bark cracking viroid (CBCVd) is a serious threat, which spreads rapidly within hop gardens. In this study, we employed comprehensive transcriptome analyses to dissect host-viroid interactions and identify gene expression changes that are associated with disease development in hop. Our analysis revealed that CBCVd-infection resulted in the massive modulation of activity of over 2000 genes. Expression of genes associated with plant immune responses (protein kinase and mitogen-activated protein kinase), hypersensitive responses, phytohormone signaling pathways, photosynthesis, pigment metabolism, protein metabolism, sugar metabolism, and modification, and others were altered, which could be attributed to systemic symptom development upon CBCVd-infection in hop. In addition, genes encoding RNA-dependent RNA polymerase, pathogenesis-related protein, chitinase, as well as those related to basal defense responses were up-regulated. The expression levels of several genes identified from RNA sequencing analysis were confirmed by qRT-PCR. Our systematic comprehensive CBCVd-responsive transcriptome analysis provides a better understanding and insights into complex viroid-hop plant interaction. This information will assist further in the development of future measures for the prevention of CBCVd spread in hop fields.

Keywords

genetske transformacije;viroid razpokanosti skorje agrumov;CBCVd;huda viroidna zakrnelost hmelja;humulus lupulus;hmelj;transkriptomska analiza;različno izraženi geni;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 577.2
COBISS: 9078905 Link will open in a new window
ISSN: 1999-4915
Views: 176
Downloads: 65
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Other data

Secondary language: Slovenian
Secondary keywords: genetske transformacije;viroid razpokanosti skorje agrumov;CBCVd;huda viroidna zakrnelost hmelja;Humulus lupulus;hmelj;transkriptomska analiza;različno izraženi geni;
Type (COBISS): Article
Pages: str. 1-19
Volume: ǂVol. ǂ10
Issue: ǂIss. ǂ10: 570
Chronology: 2018
DOI: 10.3390/v10100570
ID: 13689600