Modulation of Campylobacter jejuni motility, adhesion to polystyrene surfaces, and invasion of INT407 cells by quorum-sensing inhibition

Katarina Šimunović (Author), Dina Ramić (Author), Changyun Xu (Author), Sonja Smole Možina (Author)

Abstract

Campylobacter jejuni is a major foodborne pathogen, and the LuxS-mediated quorum-sensing (QS) system influences its motility, biofilm formation, invasion, host colonization, and virulence. QS therefore represents a target for the control of C. jejuni. The aim of this study was to investigate the correlation of QS inhibition with changes in C. jejuni motility, adhesion to polystyrene surfaces, and adhesion to and invasion of INT407 cells. This was achieved by studying (i) the luxS-deficient mutant and (ii) treatment of C. jejuni with 20 natural extracts as six essential oils, 11 ethanolic extracts, and three pure compounds. Compared to the wild-type, the ∆luxS mutant showed decreased motility, adhesion to polystyrene surfaces, and invasion of INT407 cells. The anti-QS effects of the treatments (n = 15/20) were assayed using Vibrio harveyi BB170 bioluminescence. Moderate positive correlation was shown between C. jejuni QS reduction and reduced motility (τ = 0.492, p = 0.024), adhesion to polystyrene surfaces (τ = 0.419, p = 0.008), and invasion (r = 0.394, p = 0.068). The best overall effect was achieved with a Sedum rosea (roseroot) extract, with 96% QS reduction, a 1.41 log (96%) decrease in adhesion to polystyrene surfaces, and an 82% decrease in invasion. We show that natural extracts can reduce motility, adhesion to polystyrene surfaces, and invasion of INT407 cells by C. jejuni through modulation of the LuxS (QS) system.

Keywords

patogeni mikroorganizmi;Campylobacter jejuni;protimikrobne snovi;rastlinski izvlečki;medcelično signaliziranje;zaznavanje kvoruma;gibljivost;adhezija;

Data

Language:	English
Year of publishing:	2020
Typology:	1.01 - Original Scientific Article
Organization:	UL BF - Biotechnical Faculty
UDC:	579.22/.26:547.9:615.33
COBISS:	5147768
ISSN:	2076-2607
Views:	198
Downloads:	72
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	patogeni mikroorganizmi;Campylobacter jejuni;protimikrobne snovi;rastlinski izvlečki;medcelično signaliziranje;zaznavanje kvoruma;gibljivost;adhezija;
Type (COBISS):	Article
Pages:	str. 1-14
Volume:	ǂVol. ǂ8
Issue:	ǂiss. ǂ1
Chronology:	2020
DOI:	10.3390/microorganisms8010104
ID:	13729659