a systematic Study of different liposomal formulations
Nina Kostevšek (Author), Calvin C. L. Cheung (Author), Igor Serša (Author), Mateja Erdani-Kreft (Author), Ilaria Monaco (Author), Mauro Comes Franchini (Author), Janja Vidmar (Author), Wafa T. Al-Jamal (Author)

Abstract

The majority of the clinically approved iron oxide nanoparticles (IO NPs) used as contrast agents for magnetic resonance imaging (MRI) have been withdrawn from the market either due to safety concerns or lack of profits. To address this challenge, liposomes have been used to prepare IO-based T$_2$ contrast agents. We studied the influence of different phospholipids on the relaxivity (r$_2$) values of magneto-liposomes (MLs) containing magnetic NPs in the bilayer, where a strong correlation between the bilayer fluidity and r$_2$ is clearly shown. Embedding 5-nm IO NPs in the lipid bilayer leads to a significant improvement in their relaxivity, where r$_2$ values range from 153 ± 5 s$^{−1}$ mM$^{−1}$ for DPPC/cholesterol/DSPE-PEG (96/50/4) up to 673 ± 12 s$^{−1}$ mM$^{−1}$ for DOPC/DSPE-PEG (96/4), compared to “free” IO NPs with an r$_2$ value of 16 s$^{−1}$ mM$^{−1}$, measured at 9.4 T MRI scanner. In vitro MRI measurements, together with the ICP-MS analysis, revealed MLs as highly selective contrast agents that were preferentially taken up by cancerous T24 cells, which led to an improvement in the contrast and an easier distinction between the healthy and the cancerous cells. A careful selection of the lipid bilayer to prepare MLs could offer efficient MRI contrast agents, even at very low IO NP concentrations.

Keywords

liposomes;magnetic resonance imaging;iron oxide;contrast agent;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL MF - Faculty of Medicine
UDC: 537
COBISS: 14045955 Link will open in a new window
ISSN: 2079-4991
Views: 163
Downloads: 58
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Other data

Type (COBISS): Article
Pages: str. 889-1-889-18
Volume: ǂVol. ǂ10
Issue: ǂno. ǂ5
Chronology: 2020
DOI: 10.3390/nano10050889
ID: 14092793
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