Katarina Šimunović (Author), Franz Bucar (Author), Anja Klančnik (Author), Francesco Pompei (Author), Antonello Paparella (Author), Sonja Smole Možina (Author)

Abstract

The culinary herb Satureja montana, known as winter savory, is an ingredient of traditional dishes known in different parts of the world. As an ingredient of foods it has the potential to improve their safety. In this study, the herb’s activity was investigated against Campylobacter jejuni, the leading cause of the most prevalent bacterial gastroenteritis worldwide. The ethanolic extract and essential oil of the herb were chemically characterized and six pure compounds—carvacrol, thymol, thymoquinone, p-cymene, γ-terpinene, and rosmarinic acid—were chosen for further analysis. The antimicrobial activity of the ethanolic extract (MIC 250 mg/L) was 4-fold higher compared to the essential oil. Carvacrol, thymol and thymoquinone had the strongest antimicrobial effect (MIC 31.25 mg/L) and a strong synergistic activity between carvacrol and thymol was determined (FICi 0.2). Strong inhibitory effect on C. jejuni efflux pumps (2-fold inhibition) and disruption of membrane integrity (> 80% disruption) of the herb were determined as modes of action. For resistance against the herb, C. jejuni need efflux pumps, although increased resistance against this herb does not co-occur with increased efflux pump activity, as for antibiotics. This study shows the potential of a common culinary herb for the reduction of the food pathogen C. jejuni without increasing resistance.

Keywords

protimikrobno delovanje;rastlinski izvlečki;Satureja montana;izvlečki kraškega šetraja;fenolne spojine;etanolni izvlečki;minimalna inhibitorna koncentracija;patogeni mikroorganizmi;Campylobacter jejuni;izlivne črpalke;inhibitorji izlivnih črpalk;membranske izlivne črpalke;membranska integriteta;sinergija;protimikrobne snovi;karvakrol;timol;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 579.24/.26:547.9:547.56
COBISS: 13159683 Link will open in a new window
ISSN: 2304-8158
Views: 193
Downloads: 71
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Other data

Secondary language: Slovenian
Secondary keywords: protimikrobno delovanje;rastlinski izvlečki;Satureja montana;izvlečki kraškega šetraja;fenolne spojine;etanolni izvlečki;minimalna inhibitorna koncentracija;patogeni mikroorganizmi;Campylobacter jejuni;izlivne črpalke;inhibitorji izlivnih črpalk;membranske izlivne črpalke;membranska integriteta;sinergija;protimikrobne snovi;karvakrol;timol;
Type (COBISS): Article
Pages: str. 1-15
Volume: ǂVol. ǂ9
Issue: ǂiss. ǂ4
Chronology: 2020
DOI: 10.3390/foods9040537
ID: 14092802