Vito Kovač (Author), Borut Poljšak (Author), Jasmina Primožič (Author), Polona Jamnik (Author)

Abstract

Compositions of stainless steel, nickel-titanium, cobalt-chromium and β-titanium orthodontic alloys were simulated with mixtures of Fe, Ni, Cr, Co, Ti and Mo metal ions as potential oxidative stress-triggering agents. Wild-type yeast Saccharomyces cerevisiae and two mutants ∆Sod1 and ∆Ctt1 were used as model organisms to assess the cytotoxicity and oxidative stress occurrence. Metal mixtures at concentrations of 1, 10, 100 and 1000 µM were prepared out of metal chlorides and used to treat yeast cells for 24 h. Every simulated orthodontic alloy at 1000 µM was cytotoxic, and, in the case of cobalt-chromium alloy, even 100 µM was cytotoxic. Reactive oxygen species and oxidative damage were detected for stainless steel and both cobalt-chromium alloys at 1000 µM in wild-type yeast and 100 µM in the ∆Sod1 and ∆Ctt1 mutants. Simulated nickel-titanium and β-titanium alloy did not induce oxidative stress in any of the tested strains.

Keywords

ortodontski pripomočki;kovinski ioni;kvasovke;citotoksičnost;oksidativni stres;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 616.314-089.23:546.73/.76:549.261/.263:582.283.23
COBISS: 34996227 Link will open in a new window
ISSN: 1422-0067
Views: 147
Downloads: 50
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Other data

Secondary language: Slovenian
Secondary keywords: ortodontski pripomočki;kovinski ioni;kvasovke;citotoksičnost;oksidativni stres;
Type (COBISS): Article
Pages: str. 1-16
Volume: ǂVol. ǂ21
Issue: ǂiss. ǂ21
Chronology: 2020
DOI: 10.3390/ijms21217993
ID: 14373105