Matic Resnik (Author), Janez Kovač (Author), Roman Štukelj (Author), Veronika Kralj-Iglič (Author), Petr Humpolíček (Author), Ita Junkar (Author)

Abstract

Novel Extracellular Vesicles (EVs) based diagnostic techniques are promising non-invasive procedures for early stage disease detection which are gaining importance in the medical field. EVs are cell derived particles found in body liquids, especially blood, from which they are isolated for further analysis. However, techniques for their isolation are not fully standardized and require further improvement. Herein modification of polypropylene (PP) tubes by cold Atmospheric Pressure Plasma Jet (APPJ) is suggested to minimize the EVs to surface binding and thus increase EVs isolation yields. The influence of gaseous plasma treatment on surface morphology was studied by Atomic Force Microscopy (AFM), changes in surface wettability by measuring the Water Contact Angle (WCA), while surface chemical changes were analyzed by X-Ray Photoelectron Spectroscopy (XPS). Moreover, PP tubes from different manufacturers were compared. The final isolation yields of EVs were evaluated by flow cytometry. The results of this study suggest that gaseous plasma treatment is an intriguing technique to uniformly alter surface properties of PP tubes and improve EVs isolation yields up to 42%.

Keywords

atmospheric pressure plasma jets (APPJs);extracellular vesicles (EVs);nanostructures;polypropylene (PP);

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL ZF - University College of Health Studies
UDC: 533
COBISS: 46629379 Link will open in a new window
ISSN: 2073-4360
Views: 149
Downloads: 97
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Type (COBISS): Article
Pages: str. 2363-1-2363-13
Volume: ǂVol. ǂ12
Issue: ǂno. ǂ10
Chronology: 2020
DOI: 10.3390/polym12102363
ID: 14381854