magistrsko delo
Monika Nedveš (Author), Aleš Podgornik (Mentor), Igor Plazl (Thesis defence commission member), Aleš Ručigaj (Thesis defence commission member)

Abstract

Bakteriofagi so virusi, ki se razmnožujejo s pomočjo infekcije specifičnih bakterij in jih lahko uporabljamo za zdravljenje bakterijskih okužb. V industriji bakteriofage proizvajajo v šaržnih bioreaktorjih, ker pa v prihodnosti pričakujemo čedalje večje zanimanje zaradi vse pogostejše odpornosti na antibiotike, je kontinuirno gojenje dobra alternativa trenutni obliki proizvodnje; hkrati pa je za učinkovito zdravljenje pomembno tudi poznavanje hitrosti razmnoževanja bakteriofagov. Cellstat je kontinuirni mešalni sistem, sestavljen iz dveh povezanih bioreaktorjev, skozi katera teče konstanten tok medija. V prvem bioreaktorju gojimo bakterijsko kulturo brez prisotnosti bakteriofagov in tako vzdržujemo konstantno fiziološko stanje bakterije. V drugem bioreaktorju gojimo bakteriofage, katerim s konstantnim pretokom dovajamo bakterije iz prvega bioreaktorja. Z nižanjem volumna medija v drugem bioreaktorju uravnavamo hitrost redčenja. S spremembo hitrosti redčenja smo vplivali na hitrost razmnoževanja bakteriofagov, kar lahko opišemo kot fitnes bakteriofagov – ki poveže tri ključne rastne parametre: brstno število, latentno periodo in adsorpcijsko konstantno. Z višanjem hitrosti redčenja v drugem bioreaktorju med samo proizvodnjo bakteriofagov dobimo bakteriofage z višjim fitnesom. Bakteriofagi se na nove pogoje prilagodijo tako, da se jim poveča brstno število in skrajša latentna perioda. Na samo razmnoževanje močno vpliva tudi fiziološko stanje bakterije, pri kateri gojimo bakteriofage, in le-ta vpliva na to, do kolikšne mere je možna prilagoditev bakteriofaga.

Keywords

bakterije;E. coli;bakteriofagi;bakteriofag T4;gojenje bakteriofagov;kontinuirni sistem;magistrska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [M. Nedveš]
UDC: 66.098:578.347(043.2)
COBISS: 96252675 Link will open in a new window
Views: 152
Downloads: 39
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Other data

Secondary language: English
Secondary title: Characterization of bacteriophage production in continuous stirred tank system
Secondary abstract: Bacteriophages are viruses that multiply through the infection of specific bacteria and can be used to treat bacterial infections. In the industry, bacteriophages are produced in batch bioreactors, but because we expect more and more interest in the future, due to increasing antibiotic resistance, continuous cultivation is a good alternative to the current form of production. Cellstat is a continuous stirred tank system consisting of two connected bioreactors with constant media flow. In the first bioreactor, we cultivated bacterial culture without presence of bacteriophages and maintained constant bacterial physiological state. In the second bioreactor, bacteriophages are propagated with constant flow of bacteria from first bioreactor. The dilution rate is regulated by lowering the volume of the medium in the second bioreactor. By changing the dilution rate, we regulate the bacteriophage reproduction, which can be described as bacteriophage fitness and connects three key growth parameters: burst size, latent period and adsorption constant. By increasing dilution rate in the second bioreactor during bacteriophage production, bacteriophages with higher fitness are obtained. Bacteriophages adapt to new conditions with increased burst size and shorter latent period. Reproduction itself is also strongly depended on the physiological state cultivation conditions of bacteria and this affects the extent to which bacteriophage adaptation is possible.
Secondary keywords: bacteriophages;continuous system;T4;E. coli;
Type (COBISS): Master's thesis/paper
Study programme: 1000376
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, smer Kemijsko inženirstvo
Pages: 55 str.
ID: 14427201