Metka Lenassi (Author), Janja Zajc (Author), Cene Gostinčar (Author), Alenka Gorjan (Author), Nina Gunde-Cimerman (Author), Ana Plemenitaš (Author)

Abstract

We report the first identification and characterisation of the glycerol-3-phosphate dehydrogenase (GPD) genes from extremely halophilic fungi. The black ascomycetous yeast Hortaea werneckii and the non-melanised basidiomycetous fungus Wallemia ichthyophaga inhabit similar hypersaline environments, yet they have two different strategies of haloadaptation throughGpd1-regulated glycerol synthesis. The extremely halotolerant H. werneckii codes for two salt-inducible GPD1 genes that show similar gene transcription regulation and have 98% amino-acid sequence identity between paraloguesč however, they have distinct effects when expressed heterologouslyin Saccharomyces cerevisiae gpd mutants. Only the HwGpd1B isoform complements the function of Gpd in the gpd1 mutant, whereas none of the Gpd1 isoforms can rescue the salt sensitivity of the gpd1gpd2 double mutant. The obligate halophile W. ichthyophaga codes for only one GPD1 orthologue, the transcription of which is less affected by salt when compared to the H. werneckii homologues. Heterologous expression of WiGPD1 in S. cerevisiae recovers halotolerance of the gpd1 and gpd1gpd2 mutant strains, which is probably due to the overall high amino-acid similarity of the Gpd1 protein in W. ichthyophaga and S. cerevisiae. Phylogenetic analysis of amino-acid sequences reveals that the evolutionary origins of all of these three novel enzymes correspond to the phylogeny of the fungal species from which the genes were identified.

Keywords

black yeast;glycerol;glycerol-3-phosphate dehydrogenase;halophilic fungi;HOG pathway;osmoadaptation;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 577.2
COBISS: 2396495 Link will open in a new window
ISSN: 1878-6146
Views: 1004
Downloads: 307
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Other data

Secondary language: English
Type (COBISS): Not categorized
Pages: str. 959-970
Volume: ǂVol. ǂ115
Issue: ǂissue ǂ10
Chronology: 2011
DOI: 10.1016/j.funbio.2011.04.001
ID: 1446627