Vanja Miljanić (Author), Jernej Jakše (Author), Urban Kunej (Author), Denis Rusjan (Author), Andreja Škvarč (Author), Nataša Štajner (Author)

Abstract

Diseases caused by viruses and virus-like organisms are one of the major problems in viticulture and grapevine marketing worldwide. Therefore, rapid and accurate diagnosis and identification is crucial. In this study, we used HTS of virus- and viroid-derived small RNAs to determine the virome status of Slovenian preclonal candidates of autochthonous and local grapevine varieties (Vitis vinifera L.). The method applied to the studied vines revealed the presence of nine viruses and two viroids. All viral entities were validated and more than 160 Sanger sequences were generated and deposited in NCBI. In addition, a complete description into the co-infections in each plant studied was obtained. No vine was found to be virus- and viroid-free, and no vine was found to be infected with only one virus or viroid, while the highest number of viral entities in a plant was eight.

Keywords

Vitis vinifera;preclonal candidates;HTS;viruses;viroids;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 634.8
COBISS: 98211843 Link will open in a new window
ISSN: 1664-302X
Views: 287
Downloads: 97
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Other data

Secondary language: Slovenian
Secondary keywords: vinska trta;sorte;klonska selekcija;predklonski kandidati;sekvenciranje;virusi;viroidi;
Type (COBISS): Article
Pages: 11 str.
Issue: ǂVol. ǂ13
Chronology: 2022
DOI: 10.3389/fmicb.2022.830866
ID: 14586106