Urban Kunej (Author), Jernej Jakše (Author), Sebastjan Radišek (Author), Nataša Štajner (Author)

Abstract

RNA interference is an evolutionary conserved mechanism by which organisms regulate the expression of genes in a sequence-specific manner to modulate defense responses against various abiotic or biotic stresses. Hops are grown for their use in brewing and, in recent years, for the pharmaceutical industry. Hop production is threatened by many phytopathogens, of which Verticillium, the causal agent of Verticillium wilt, is a major contributor to yield losses. In the present study, we performed identification, characterization, phylogenetic, and expression analyses of three Argonaute, two Dicer-like, and two RNA-dependent RNA polymerase genes in the susceptible hop cultivar Celeia and the resistant cultivar Wye Target after infection with Verticillium nonalfalfae. Phylogeny results showed clustering of hop RNAi proteins with their orthologues from the closely related species Cannabis sativa, Morus notabilis and Ziziphus jujuba which form a common cluster with species of the Rosaceae family. Expression analysis revealed downregulation of argonaute 2 in both cultivars on the third day post-inoculation, which may result in reduced AGO2-siRNA-mediated posttranscriptional gene silencing. Both cultivars may also repress ta-siRNA biogenesis at different dpi, as we observed downregulation of argonaute 7 in the susceptible cultivar on day 1 and downregulation of RDR6 in the resistant cultivar on day 3 after inoculation.

Keywords

Humulus lupulus;Verticillium nonalfalfae;verticilijska uvelost;glivična okužba;interferenca RNA;interakcija rastlina-patogen;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 577.2
COBISS: 60443139 Link will open in a new window
ISSN: 1422-0067
Views: 154
Downloads: 57
Average score: 0 (0 votes)
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Other data

Secondary language: Slovenian
Secondary keywords: Humulus lupulus;Verticillium nonalfalfae;verticilijska uvelost;glivična okužba;interferenca RNA;interakcija rastlina-patogen;
Type (COBISS): Article
Pages: str. 1-16 (4224)
Volume: ǂVol. ǂ22
Issue: ǂiss. ǂ8
Chronology: 2021
DOI: 10.3390/ijms22084224
ID: 14641673