magistrsko delo
Tomaž Pšeničnik (Author), Irena Drevenšek Olenik (Mentor), Lea Spindler (Co-mentor)

Abstract

Optični metodi dinamično sipanje svetlobe (DLS) in diferenčna dinamična mikroskopija (DDM) sta primerni eksperimentalni tehniki za zaznavanje submikronskih delcev v različnih suspenzijah. V našem primeru so to vodne suspenzije z gvaninom bogatih oligonukleotidov DNK, ki ob prisotnosti primerne koncentracije kationov, kot sta recimo Na+ ali K+, tvorijo daljše strukture imenovane G-žičke. Metodi sta osnovani na sipanju svetlobe in izračunu avtokorelacijske funkcije intenzitete svetlobe zaznane na fotopomnoževalki ali zelo hitri kameri. Kljub temu, da je princip merjenja pri omenjenih metodah različen, se rezultati - na podlagi primernega matematičnega modela - med seboj dopolnjujejo in so primerljivi. Z navedenima metodama smo določili difuzijske koeficiente sipalcev suspenzij, iz katerih smo ugotovili, katere mutacije začetnega oligonukleotida d(G2AG4 AG2) tvorijo G-žičke ter kolikšna je dolžina teh žičk. Zaznali smo G-žičke različnih velikosti, katerih efektivne dolžine so v razponu od L min ⠼ 20 nm do L max ⠼ 140 nm.

Keywords

oligonukleotidi DNK;G-kvarteti;G-kvadrupleksi;G-žičke;Brownovo gibanje;dinamično sipanje svetlobe;diferenčna dinamična mikroskopija;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL FMF - Faculty of Mathematics and Physics
Publisher: [T. Pšeničnik]
UDC: 544
COBISS: 99751683 Link will open in a new window
Views: 175
Downloads: 36
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Other data

Secondary language: English
Secondary title: Characterization of formation of guanosine nanowires by light scattering methods
Secondary abstract: Optical methods, such as dynamic light scattering (DLS) and differential dynamic microscopy (DDM) are suitable experimental techniques for detecting submicron particles in different suspensions. In our case, these are aqueous suspensions of guanine-rich oligonucleotides that in the presence of a suitable concentration of cations, such as Na+ or K+, form longer structures called G-wires. The methods are based on light scattering and the calculation of the autocorrelation function of the perceived light intensity on a photomultiplier or a high-speed camera. Despite the fact, that the principle of measurement in the two mentioned methods is different, the results - based on a suitable mathematical model - complement each other and are comparable. With both methods, we determined the diffusion coefficients of the suspension, from which we deduced, which mutation of the initial oligonucleotide d(G2AG4AG2) form G-wires and what is the length of these wires. We detected G-wires of different sizes, whose effective lengths range from L min ⠼ 20 nm to L max ⠼ 140 nm.
Secondary keywords: DNA oligonucleotides;G-quartets;G-quadruplexes;G-wires;Brownian motion;dynamic light scattering;differential dynamic microscopy;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za matematiko in fiziko, Oddelek za fiziko
Pages: 87 str.
ID: 14687905