magistrsko delo
Anže Šlibar (Author), Jasna Dolenc Koce (Reviewer), Nataša Štajner (Mentor), Marko Flajšman (Co-mentor)

Abstract

Raziskave že desetletja uvrščajo navadno konopljo (Cannabis sativa L.) med hitro rastoče, nezahtevne, hiperakumulativne rastline. Pripisujejo ji visoko stopnjo privzema težkih kovin, medtem ko geni, odzivni na prisotnost potencialno toksičnih kovin, pri navadni konoplji še niso sekvencirani in anotirani, s tem pa tudi ni dodobra raziskana njihova potencialna vključenost v privzem kovin. Ta raziskava skuša zapolniti vrzel nasprotujočih si ali nepopolnih podatkov v literaturi in preučuje vključenost genov GSR 1, PLD α, PDR 12 in HMA 4 v metabolizmu potencialno toksičnih kovin v navadni konoplji. Raziskava temelji na lastnih eksperimentalnih podatkih ter analizah v kontroliranem lončnem poskusu, pri čemer smo preučevali vključenost genov na dve potencialno toksični kovini, svinec in kadmij, pri dveh sortah navadne konoplje, Futura 75 in Tisza. Z načrtovanjem začetnih oligonukleotidov na podlagi medvrstno ohranjenih genskih zaporedij - ortologov v že anotiranih vrstah, kot so Arabidopsis thalinana, Brassica juncea, Lactuca sativa, Triticum urartu ter Vitis vinifera, pa raziskava nudi tudi posredni vpogled v podobnost nukleotidnih zaporedij tovrstnih genov v navadni konoplji.

Keywords

Cannabis sativa L.;identifikacija;karakterizacija;stresni odziv;težke kovine;molekularna genetika;začetni oligonukleotidi;real-time qPCR;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [A. Šlibar]
UDC: 601.4:577.21:633.522:549.25(043.2)
COBISS: 102801923 Link will open in a new window
Views: 145
Downloads: 27
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Other data

Secondary language: English
Secondary title: Identification and characterization of Cannabis sativa L. genes responsive to potentially toxic metals
Secondary abstract: Research has classified industrial hemp (Cannabis sativa L.) as a fast-growing, undemanding, hyperaccumulative plant for decades. It is also attributed a high level of heavy metal uptake, while genes responsive to the presence of potentially toxic metals in industrial hemp have not been sequenced and annotated, and their involvement in their uptake is not well understood. The study tries to fill the gap of conflicting or incomplete data in literature, and investigates the involvemenet of GSR 1, PLD α, HSP70, PDR 12, and HMA 4 genes in the response of industrial hemp to potentially toxic metals. The study is based on our own experimental data and analyses in a controlled pot experiment, whereby we evaluated the involvement of genes in the response to two potentially toxic metals, lead and cadmium, in two separate varieties of hemp, Futura 75 and Tisza. By designing the initial oligonucleotides on the basis of interspecific preserved gene sequences - orthologs in already annotated species such as Arabidopsis thalinana, Brassica juncea, Lactuca sativa, Triticum urartu and Vitis vinifera, the study also offers an indirect insight into the similarity of nucleotide sequences of these genes in industrial hemp.
Secondary keywords: identification;characterization;stress response;heavy metals;molecular genetics;primers;real-time qPCR;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: X, 51 str., [23] str. pril.
ID: 14918320