Martina Grdiša (Author), Zlatko Šatović (Author), Zlatko Liber (Author), Jernej Jakše (Author), Filip Varga (Author), Renata Erhatić (Author), Siniša Srečec (Author)

Abstract

Hop (Humulus lupulus L.) is used in the brewing industry as a source of compounds responsible for the bitterness, aroma, and preservative properties of beer. In this study, we used microsatellite markers to investigate genetic diversity and genetic differentiation of wild hop populations sampled in the northwestern part of Croatia. Analysis of 12 microsatellite loci revealed high diversity and weak population differentiation among wild hop populations. A total of 152 alleles were determined with an average of 12.67 alleles per locus. Observed heterozygosity ranged from 0.689 to 0.839 (average 0.767) and expected heterozygosity ranged from 0.725 to 0.789 (average 0.760). A total of 38 private alleles were detected. The data suggest that H. lupulus populations are not affected by recent bottlenecks. The degree of genetic differentiation among populations was low and not significant for most pairwise F$_{ST}$ values, except for the pair of geographically most distant populations. The results did not indicate the existence of genetic structure among the sampled populations. The high genetic diversity and low differentiation among populations, combined with the absence of isolation by distance, indicate the existence of substantial gene flow among wild hop populations. Therefore, extensive sampling per population is clearly required to assess the genetic diversity of hop populations. Sampling strategies involving sampling across a large number of localities represented by only a few samples could lead to erroneous conclusions.

Keywords

genetska raznolikost;genetski viri;Humulus lupulus;divji hmelj;Hrvaška;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 631
COBISS: 74705923 Link will open in a new window
ISSN: 2076-3417
Views: 111
Downloads: 33
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Other data

Secondary language: Slovenian
Secondary keywords: genetska raznolikost;genetski viri;Humulus lupulus;divji hmelj;Hrvaška;
Type (COBISS): Article
Pages: Str. 1-14
Volume: ǂVol. ǂ11
Issue: ǂiss. ǂ14, art. 6484
Chronology: 2021
DOI: 10.3390/app11146484
ID: 14956883