Katja Goričar (Author), Marija Holcar (Author), Nina Mavec (Author), Viljem Kovač (Author), Metka Lenassi (Author), Vita Dolžan (Author)

Abstract

Malignant mesothelioma (MM) is characterized by poor prognosis and short survival. Extracellular vesicles (EVs) are membrane-bound particles released from cells into various body fluids, and their molecular composition reflects the characteristics of the origin cell. Blood EVs or their miRNA cargo might serve as new minimally invasive biomarkers that would enable earlier detection of MM or treatment outcome prediction. Our aim was to evaluate miRNAs enriched in serum EVs as potential prognostic biomarkers in MM patients in a pilot longitudinal study. EVs were isolated from serum samples obtained before and after treatment using ultracentrifugation on 20% sucrose cushion. Serum EV-enriched miR-103-3p, miR-126-3p and miR-625-3p were quantified using qPCR. After treatment, expression of miR-625-3p and miR-126-3p significantly increased in MM patients with poor treatment outcome (p = 0.012 and p = 0.036, respectively). A relative increase in miR-625-3p expression after treatment for more than 3.2% was associated with shorter progression-free survival (7.5 vs. 19.4 months, HR = 3.92, 95% CI = 1.20-12.80, p = 0.024) and overall survival (12.5 vs. 49.1 months, HR = 5.45, 95% CI = 1.06-28.11, p = 0.043) of MM patients. Bioinformatic analysis showed enrichment of 33 miR-625-3p targets in eight biological pathways. Serum EV-enriched miR-625-3p could therefore serve as a prognostic biomarker in MM and could contribute to a more personalized treatment.

Keywords

mezoteliom;zunajcelični vezikli;miR-625;mesothelioma;extracellular vesicles;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL MF - Faculty of Medicine
UDC: 616-006
COBISS: 80910851 Link will open in a new window
ISSN: 2075-4426
Views: 91
Downloads: 38
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Other data

Secondary language: Slovenian
Secondary keywords: mezoteliom;zunajcelični vezikli;miR-625;
Type (COBISS): Article
Pages: str. 1-18
Volume: ǂVol. ǂ11
Issue: ǂno. ǂ10
Chronology: 2021
DOI: 10.3390/jpm11101014
ID: 15098223