Zmanjšana občutljivost bakterij, izoliranih iz terapevtskih pripomočkov za večkratno uporabo proti razkužilom

(magistrsko delo)

Barbara Turković (Author), Urška Rabuza (Mentor), Darja Duh (Co-mentor), Mojca Cimerman (Co-mentor)

Abstract

V zadnjih letih se je povečalo število okužb, povezanih z zdravstvom, katerih glavni vir so bolniki in površine ter različni pripomočki. Glede na problem odpornosti bakterij proti antibiotikom in potencialni odpornosti proti razkužilom je potrebnih več raziskav, s katerimi bi lahko izdelali ustrezne protokole za uporabo biocidnih proizvodov. Namen zaključnega dela je bil določiti, ali je prisotna zmanjšana občutljivost bakterij, izoliranih iz terapevtskih pripomočkov za večkratno uporabo proti razkužilom. Podatke smo pridobili s pomočjo eksperimenta, ki je potekal v laboratoriju. Z eksperimentom smo določili občutljivost bakterij na razkužila. Občutljivost je količinsko opredeljena z MIC, ki ga lahko definiramo kot najmanjšo koncentracijo razkužila, ki je potrebna za preprečevanje rasti kulture.Bakterije, ki smo jih analizirali, niso kazale na zvišano raven odpornosti oziroma zmanjšano občutljivost. Koncentracija, ki je zavirala rast bakterij, je znašala med 6,25 % in 12,5 %. Ker se razkužila, ki smo jih testirali, uporabljajo v koncentracijah, v katerih so, torej brez redčenja, sklepamo, da že nižje koncentracije zadostujejo za zaviranje in uničenje bakterij. Lahko sklepamo, da so pri Incidin Liquidu potrebne manjše doze učinkovine kot pri Sani Cloth. Neustrezno razkuženi pripomočki so zlahka vir okužbe. Potrebno je standardizirati protokole razkuževanja in redno spremljati odpornost bakterij na sredstva, ki se uporabljajo.

Keywords

občutljivost;razkužila;alkohol;MIC;bakterije;

Data

Language:	Slovenian
Year of publishing:	2022
Typology:	2.09 - Master's Thesis
Organization:	UM FZV - Faculty of Health Sciences
Publisher:	[B. Turković]
UDC:	579.8:615.28(043.2)
COBISS:	110965507
Views:	113
Downloads:	17
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Reduced sensitivity of bacteria isolated from reusable therapeutic devices against disinfectants
Secondary abstract:	In recent years, there has been an increase in the number of health-related infections whose main source is patients and surfaces and various aids. Thus the problem of bacterial resistance to antibiotics and potential resistance to disinfectants, more research is needed to develop appropriate protocols for the use of biocidal products. The purpose of this work was to determine whether there is a reduced susceptibility of bacteria isolated from reusable therapeutic devices against disinfectants.The data were obtained with the help of an experiment conducted in the laboratory. The susceptibility of bacteria to disinfectants was determined experimentally. Susceptibility is quantified by the MIC, which can be defined as the minimum concentration of disinfectant required to prevent culture growth.The bacteria we analyzed did not indicate an increased level of resistance or decreased sensitivity. The concentration inhibiting bacterial growth ranged between 6.25% and 12.5%. Since the disinfectants we tested are used in concentrations in which they are, ie without dilution, we conclude that even lower concentrations are sufficient to inhibit and destroy bacteria. We can conclude that Incidin Liquid requires lower doses of the active ingredient than Sani Cloth.Inadequately disinfected devices are easily a source of infection. Disinfection protocols need to be standardized and bacterial resistance to the agents used should be monitored regularly.
Secondary keywords:	sensitivity;disinfectants;alcohol;MIC;bacteria;Disinfection;Anti-bacterial agents;Microorganisms;Razkuževanje;Antibakterijska sredstva;Mikroorganizmi;
Type (COBISS):	Master's thesis/paper
Thesis comment:	Univ. v Mariboru, Fak. za zdravstvene vede
Pages:	V, 49 f.
ID:	15193732