Vanja Miljanić (Author), Jernej Jakše (Author), Denis Rusjan (Author), Andreja Škvarč (Author), Nataša Štajner (Author)

Abstract

Metagenomic approaches used for virus diagnostics allow for rapid and accurate detection of all viral pathogens in the plants. In order to investigate the occurrence of viruses and virus-like organisms infecting grapevine from the Ampelographic collection Kromberk in Slovenia, we used Ion Torrent small RNA sequencing (sRNA-seq) and the VirusDetect pipeline to analyze the sRNA-seq data. The used method revealed the presence of: Grapevine leafroll-associated virus 1 (GLRaV-1), Grapevine leafroll-associated virus 2 (GLRaV-2), Grapevine leafroll-associated virus 3 (GLRaV-3), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine fanleaf virus (GFLV) and its satellite RNA (satGFLV), Grapevine fleck virus (GFkV), Grapevine rupestris vein feathering virus (GRVFV), Grapevine Pinot gris virus (GPGV), Grapevine satellite virus (GV-Sat), Hop stunt viroid (HSVd), and Grapevine yellow speckle viroid 1 (GYSVd-1). Multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for validation of sRNA-seq predicted infections, including various combinations of viruses or viroids and satellite RNA. mRT-PCR could further be used for rapid and cost-effective routine molecular diagnosis, including widespread, emerging, and seemingly rare viruses, as well as viroids which testing is usually overlooked.

Keywords

vitis vinifera;grapevine;virome;metagenomic analysis;viruses;sRNA-seq;molecular diagnostics;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 577.2
COBISS: 106521859 Link will open in a new window
ISSN: 1999-4915
Views: 292
Downloads: 86
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Other data

Secondary language: Slovenian
Secondary keywords: vinska trta;virusne bolezni;virusi;viroidi;molekularna diagnostika;metagenomska analiza;
Type (COBISS): Article
Pages: 12 str.
Volume: ǂVol. ǂ14
Issue: ǂIss. ǂ5 (921)
Chronology: 2022
DOI: 10.3390/v14050921
ID: 15209478