Knee and peri-knee tissues of post mortem donors are strategic sources of Mesenchymal stem/stromal Cells for regenerative procedures

Gregor Haring (Author), Janja Zupan (Author)

Abstract

Tissues of post mortem donors represent valuable alternative sources for the isolation of primary cells with mesenchymal stem/stromal cell (MSC)-like properties. However, the properties of primary cells derived from different tissues and at different post mortem times are poorly recognized. Here, we aim to identify the optimal tissue source between three knee and peri-knee tissues for the isolation of primary cells with MSC-like properties, and to define the influence of the time post mortem on the properties of these cells. We harvested tissues from subchondral bone marrow, synovium and periosteum from 32 donors at various post mortem times. Primary cells were evaluated using detailed in vitro analyses, including colony formation, trilineage differentiation, immunophenotyping and skeletal stem cell marker-gene expression profiling. These data show that the primary cells with MSC-like properties isolated from these three tissues show no differences in their properties, except for higher expression of CD146 in bone-marrow cells. The success rate of the primary cell isolation is dependent on the post mortem time. However, synovium and periosteum cells isolated more than 48 h post mortem show improved osteogenic and chondrogenic potential. This study suggests that knee and peri-knee tissues from donors even 3 days post mortem are strategic sources of MSCs for regenerative procedures.

Keywords

mezenhimske matične/stromalne celice;darovalci ob smrti skeletnih matičnih celic;koleno;perikolenska tkiva;kostni mozeg;sinovij;periosteum;in vitro analiza;označevalci človeških skeletnih matičnih celic;mesenchymal stem/stromal cells;post mortem donors;knee;peri-knee tissues;bone and bone marrow;synovium;in vitro analysis;human skeletal stem cell markers;

Data

Language:	English
Year of publishing:	2022
Typology:	1.01 - Original Scientific Article
Organization:	UL FFA - Faculty of Pharmacy
UDC:	602.9:576.7:611.728.3
COBISS:	103163395
ISSN:	1422-0067
Views:	59
Downloads:	32
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	Matične celice;Mezenhimske matične celice;Tkiva;
Type (COBISS):	Article
Pages:	str. 1-21
Volume:	ǂVol. ǂ23
Issue:	ǂiss. ǂ6
Chronology:	2022
DOI:	10.3390/ijms23063170
ID:	15702047