Razvoj in učinkovitost dveh okolju prijaznih inovativnih načinov zatiranja koruznega hrošča (Diabrotica virgifera virgifera LeConte [Coleoptera: Chrysomelidae])

doktorska disertacija

Špela Modic (Author), Stanislav Trdan (Mentor), Jaka Razinger (Co-mentor)

Abstract

Koruzni hrošč (Diabrotica virgifera virgifera LeConte) je gospodarsko pomemben škodljivec koruze v Evropi in v Severni Ameriki, ki povzroča zelo velike izgube pridelka. V pričujoči disertaciji smo v triletnem poljskem poskusu (2016–2018), ki je potekal na dveh pedoklimatsko različnih lokacijah z naravno gostoto populacije koruznega hrošča, preučili učinkovitost entomopatogenih ogorčic (EO) vrste Heterorhabditis bacteriophora Poinar kot okolju prijaznega načina zatiranja ličink koruznega hrošča. Pri tem smo sočasno primerjali učinkovitost EO z učinkovitostjo dveh pogosto uporabljenih konvencionalnih insekticidov na podlagi teflutrina (Force 1,5 G) iz skupine sintetičnih piretriodov in tiakloprida (Sonido FS 400) iz skupine neonikotinoidov za zatiranje talnih škodljivcev v koruzi. Razvili smo manjši prenosni sistem za vnos EO v tla ob setvi koruze. Ugotovili smo, da je zatiranje ličink koruznega hrošča z vrsto H. bacteriophora značilno vplivalo na manjše izleganje hrošča in da je enako učinkovito kot uporaba granuliranega insekticida teflutrina. Nadalje smo preučili toksičnost proteinskih kompleksov na podlagi egerolizinov OlyA6/PlyB, PlyA2/PlyB in EryA/PlyB iz gliv rodu ostrigarjev (Pleurotus spp.) za koruznega hrošča. Ugotovili smo, da je kompleks OlyA6/PlyB značilno povečal smrtnost odraslih hroščev. Menimo, da je uporaba EO okolju prijazna rešitev za zatiranje koruznega hrošča in da bi kompleksi na podlagi egerolizinov lahko prispevali k razvoju novih bioinsekticidov za zatiranje ciljnih rastlinskih škodljivcev.

Keywords

koruza;koruzni hrošč;preplavno biotično varstvo;entomopatogene ogorčice;Heterorhabditis bacteriophora;egerolizini;bukov ostrigar;Pleurotus;

Data

Language:	Slovenian
Year of publishing:	2022
Typology:	2.08 - Doctoral Dissertation
Organization:	UL BF - Biotechnical Faculty
Publisher:	[Š. Modic]
UDC:	632.76:632.937.1:595.161:582.284.514(043.3)
COBISS:	110806531
Views:	149
Downloads:	31
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Development and efficacy of two environmentally friendly innovative management strategies against western corn rootworm (Diabrotica virgifera virgifera LeConte [Coleoptera: Chrysomelidae])
Secondary abstract:	The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, is an economically important pest of maize in Europe and North America, causing considerable yield losses. This dissertation presents results of a three-year field study (2016–2018) conducted in two different pedoclimatic regions naturally infested with a WCR population. We studied the effectiveness of entomopathogenic nematodes (EPN) Heterorhabditis bacteriophora Poinar as an environmentally friendly method against WCR larvae. Concurrently, we compared the effectiveness of EPN to two most commonly used insecticides based on the active ingredient tefluthrin (Force 1,5 G) from the group of synthetic pyrethriods, and the active ingredient thiacloprid (Sonido FS 400) from the group of neonicotinoids, against soil pests in maize. We developed a special system for EPN application into the soil when sowing maize. We found that the treatment with H. bacteriophora significantly affects the hatching of the beetle and was as effective as a tefluthrin treatment. We further evaluated the toxicities of the aegerolysin-based protein complexes OlyA6/PlyB, PlyA2/PlyB and EryA/PlyB from the oyster mushroom (Pleurotus spp.) to adult WCR. We found that the OlyA6/PlyB complex significantly increasing the beetle mortality. We believe that EPN provide an environmentally friendly solution to WCR control and that the aegerolysin-based protein complexes may contribute to the development of new bioinsecticides to control target plant pest.
Secondary keywords:	maize;western corn rootwarm;inundative biological control;entomopathogenic nematodes;aegerolysin;oyster mushroom;
Type (COBISS):	Doctoral dissertation
Study programme:	0
Embargo end date (OpenAIRE):	1970-01-01
Thesis comment:	Univ. v Ljubljani, Biotehniška fak.
Pages:	XIII, 109 f.
ID:	15720970