Filip Varga (Author), Zlatko Liber (Author), Jernej Jakše (Author), Ante Turudić (Author), Zlatko Šatović (Author), Ivan Radosavljević (Author), Nina Jeran (Author), Martina Grdiša (Author)

Abstract

Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevis.) Sch. Bip.) is an outcrossing plant species (2n = 18) endemic to the eastern Adriatic coast and source of the natural insecticide pyrethrin. Due to the high repeatability and large genome (1C-value = 9.58 pg) our previous attempts to develop microsatellite markers using the traditional method were unsuccessful. Now we have used Illumina paired-end whole genome sequencing and developed a specific procedure to obtain useful microsatellite markers. A total of 796,130,142 high-quality reads (approx. 12.5× coverage) were assembled into 6,909,675 contigs using two approaches (de novo assembly and joining of overlapped pair-end reads). A total of 31,380 contigs contained one or more microsatellite sequences, of which di-(59.7%) and trinucleotide (25.9%) repeats were the most abundant. Contigs containing microsatellites were filtered according to various criteria to achieve better yield of functional markers. After two rounds of testing, 17 microsatellite markers were developed and characterized in one natural population. Twelve loci were selected for preliminary genetic diversity analysis of three natural populations. Neighbor-joining tree, based on the proportion of shared alleles distances, grouped individuals into clusters according to population affiliation. The availability of codominant SSR markers will allow analysis of genetic diversity and structure of natural Dalmatian pyrethrum populations as well as identification of breeding lines and cultivars.

Keywords

Tanacetum cinerariifolium;Dalmatian pyrethrum;Asteraceae;genomic SSRs;microsatellite markers;SSR markers;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 633
COBISS: 115302403 Link will open in a new window
ISSN: 2223-7747
Views: 47
Downloads: 23
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Other data

Secondary language: Slovenian
Secondary keywords: dalmatinski bolhač;genetika;mikrosatelitski markerji;genetska raznolikost;SSR markerji;
Type (COBISS): Article
Pages: 11 str.
Volume: ǂVol. ǂ11
Issue: ǂiss. ǂ13, art. 1778
Chronology: 2022
DOI: 10.3390/plants11131778
ID: 15907831