diplomsko delo
Sergej Praček (Author), Nataša Štajner (Mentor)

Abstract

Viroidi so skupina enoverižnih kovalentno zaprtih RNA molekul, ki jih delimo v družini Pospiviroidae in Avsunviroidae. So najmanjši rastlinski patogeni, ki na različne načine povzročijo bolezensko stanje pri gostiteljskih rastlinah (krompir, paradižnik, hmelj, vinska trta, agrumi …). Patogeneza je lahko sprožena prek interakcije med zrelo viroidno RNA molekulo in rastlinskimi proteini (strukturno specifično) ali prek malih viroidnih inferenčnih RNA molekul, ki nastanejo ob DCL procesiranju (specifično glede na nukleotidno zaporedje). Ob razumevanju delovanja viroidov so bile razvite metode za izdelavo tolerantnih rastlin na okužbo z viroidom. Največji potencial predstavljajo metode transgenze, kjer lahko z vnosom lasničnih RNA ali umetnih malih RNA preko aktivacije RNA interference zaščitimo rastline.

Keywords

biotehnologija;viroid;rastline;RNA;patogenost;metilacija;utišanje genov;konvencionalne metode;metode transgeneze;odpornost rastlin;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [S. Praček]
UDC: 606:632.38:601.4:577.21(043.2)
COBISS: 124982275 Link will open in a new window
Views: 24
Downloads: 3
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Other data

Secondary language: English
Secondary title: Researches on plant resistance to viroid infections at the molecular level
Secondary abstract: Viroids are a group of single-stranded covalently closed RNA molecules, which belong in two families Pospiviroidae and Avsunviroidae. They are the smallest plant pathogens, that can elicit disease state at host plants (potato, tomato, hops, vine, citruses …). Pathogenesis can be triggered with interaction between the mature RNA molecule and plant proteins (structurally specific) or via viroid small interfering RNA molecules, which are produced in DCL processing (nucleotide sequence specific). Understanding how viroids work, methods for viroid-tolerant plants are being developed. The greatest potential is presented by transgenesis approaches, where plants can be protected by insertion of hairpin RNA or artificial small RNA through the activation of RNA interference.
Secondary keywords: biotechnology;viroid;plants;RNA;pathogenesis;small RNA;methylation;gene silencing;plant resistance;conventional approaches;transgenic approaches;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: 1 spletni vir (1 datoteka PDF (VI, 21 f.))
ID: 16354557