magistrsko delo
Žan Hozjan (Author), Aleš Podgornik (Mentor)

Abstract

Biofilmi so skupki bakterijskih celic, pritrjeni med sabo in na podlago s pomočjo proizvedenega zunajceličnega matriksa. Bakterije tvorijo biofilme v sklopu svojih preživetvenih mehanizmov. Njihov metabolizem se upočasni, spremenita se genska ekspresija in produkcija proteinov. Te adaptacije naredijo bakterije odpornejše proti splošnim antimikrobnim terapijam, imunskemu odzivu in zdravljenju z antibiotiki. Za uspešno spopadanje z bakterijskimi biofilmi je potrebno razviti nove načine zdravljenja. Večina dosedanjih raziskav temelji na raziskavah biofilmov v stoječem mediju, kar ne predstavlja realnega kliničnega stanja. Za uspešnost raziskav, ki se ukvarjajo s škodljivimi biofilmi je potrebno vzpostaviti robusten sistem za tvorbo biofilmov v tekočem mediju, ki bo omogočal izvajanje ponovljivih eksperimentov. Da bi uspešno razumeli in imeli sposobnost apliciranja terapij v prihodnosti, pa je potrebno razumeti tudi tvorbo biofilmov pri različnih pogojih. V nalogi smo preizkusili robustnost novega bioreaktorskega sistema pri tvorbi biofilmov. V sistemu smo pri različnih pogojih uspeli vzgojiti biofilme bakterij E. coli K-12 MG1655, S. capitis in E. coli K-12 MG1655 z vstavljenim plazmidom mRFP1. Med eksperimenti smo spreminjali čas nalaganja in hitrost spiranja. Pri večini eksperimentov čas nalaganja ni imel vpliva na količino nastalega biofilma, visoke hitrosti spiranja pa so znižale gostoto biofilmskih bakterij. Pokazali smo, da je vzpostavljen sistem dovolj robusten, da dovoli manipulacijo tvorbe biofilmov pri različnih preizkušenih bakterijah.

Keywords

biotehnologija;antibiotiki;antibiotična rezistenca;biofilm;E. coli;kontinuirni cevni bioreaktor;S. capitis;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [Ž. Hozjan]
UDC: 604.4:615.33:628.353.153(043.2)
COBISS: 125651459 Link will open in a new window
Views: 15
Downloads: 7
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: English
Secondary title: Characterization of biofilms formed by various bacteria species in a continuous flow system
Secondary abstract: Biofilms are aggregates of bacterial cells, attached to each other and the surface through production of extracellular matrix. Bacteria form biofilms as part of their survival mechanisms. Their metabolism slows down and their gene expression and protein production change. These adaptations make the bacteria more resistant to antimicrobial treatments, human immune response and antibiotic therapies. In order to successfully treat bacterial biofilms in the future, it is necessary to further test and develop novel methods of treatment. Most up to date research of biofilms is conducted under zero flow confitions, which does not adequately represent the conditions of real life clinical problems. This indicates the importance of establishing a robust flow system for biofilm formation that will allow researchers to carry out future experiments. In order to better understand environmental impacts and mechanics on biofilms, it is also necessary to understand the formation of biofilms in different conditions. In the thesis, we tested the robustness of the new flow bioreactor system for biofilm formation. We managed to form the biofilms of E. coli K-12 MG1655, S. capitis and E. coli K-12 MG1655 with inserted mRFP1 plasmid. We adjusted bacterial loading time and rinsing buffer flowrate throughout the experiments. In most experiments, loading time had no effect on the amount of biofilm formed. However, higher flowrates reduced the concentrations of biofilm bacteria. We showed that the novel system is robust enough to allow replication and manipulation of biofilm formation in the various tested bacteria.
Secondary keywords: biotechnology;antibiotics;antibiotic resistance;biofilm;coiled flow inverter reactor;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: 1 spletni vir (1 datoteka PDF (IX, 49 f.))
ID: 16469452