Polymorphism analysis of the promoter of cow lactoferrin gene with PCR-RFLP and its correlation with subclinical mastitis

Chang-hong Zhao (Author), Gao-ming He (Author), Yan-liang Wang (Author), Zhao-xia Zhang (Author)

Abstract

CMT was used to detect the incidence of mastitis. One hundred twenty cows were selected and assigned into 2 groups, 60 animals in each group: control group (healthy cows), experimental group (cows with subclinical mastitis) and the relationship between cow's subclinical mastitis and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the promoter of cow lactoferrin gene was explored. The results showed that polymorphism existed in the promoter of cow lactoferrin gene, which suggested that this polymorphism could be associated with mastitis susceptibility.

Keywords

cattle;dairy cows;diseases;subclinical mastitis;molecular genetics;lactoferrin gene;PCR-RFLP;

Data

Language:	English
Year of publishing:	2008
Typology:	1.03 - Short Scientific Article
Organization:	UL BF - Biotechnical Faculty
Publisher:	Biotehniška fakulteta
UDC:	636.2
COBISS:	2415496
ISSN:	1581-9175
Parent publication:	Acta agriculturae Slovenica
Views:	861
Downloads:	213
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary title:	PCR-RFLP analiza polimorfizmov v promotorju laktoferinskega gena pri govedu in povezava s subkliničnim mastitisom
Secondary abstract:	Mastitis smo ugotavljali s na osnovi kliničnih znakov. Sto dvajset krav smo razdelili v dve skupini, 60 živali je bilo v kontrolni skupini (zdrave živali), drugih 60 pa v poskusni skupini (živali s subkliničnim mastitisom) in ugotavljali povezavo med subkliničnim mastitisom in polimorfizmom restrikcijskih fragmentov (PCR-RFLP) promotorske regije gena za laktoferin. Rezultati kažejo, da bi bil polimorfizem v promotorski regiji govejega laktoferinskega gena lahko povezan z dovzetnostjo za mastitis.
Secondary keywords:	govedo;krave;molznice;bolezni;subklinični mastitis;molekularna genetika;geni;lactoferin;PCR-RFLP;
URN:	URN:NBN:SI
Type (COBISS):	Not categorized
Pages:	str. 185-187
Volume:	ǂLetn. ǂ92
Issue:	ǂšt. ǂ2
Chronology:	2008
ID:	1734912