Rok Novak (Author), Bojana Bogovič Matijašić (Author), Irena Rogelj (Author)

Abstract

V prehrani živali se uporablja širok spekter probiotičnih mikroorganizmov, ki pripadajo mlečnokislinskim bakterijam, kvasovkam ali rodu Bacillus. V raziskavi smo ugotavljali število mikroorganizmov oziroma njihovih spor v različnih krmnih mešanicah in premiksih, ki so vsebovali komercialne probiotične pripravke Bioplus 2b, Vebac ali Biosaf. Pri bakterijskih sevih Bacillus subtilis in Bacillus licheniformis, ki smo ju osamili iz pripravka Bioplus 2b, smo ugotavljali občutljivost za antibiotike, protimikrobno aktivnost ter sposobnost preivetja v simuliranem želodčnem in črevesnem soku. Ugotovili smo, da je bilo v vseh testiranih proizvodih število probiotičnih mikroorganizmov oziroma spor manjše od deklariranega. Razlika med deklariranim in ugotovljenim številom je bila največja pri proizvodih z dodatkom pripravkov Vebac in Biosaf. Izolata B. subtilis in B. licheniformis sta občutljiva za vse izbrane antibiotike razen metronidazola. Test preživetja je pokazal, da simulirana želodčni oz. črevesni sok nimata večjega vpliva na preživetje spor, vegetativne celice obeh sevov Bacillus pa so zelo občutljive za nizke vrednosti pH in žolčne soli. B. licheniformis je inhibiral rast predvsem po Gramu pozitivnih bakterij, medtem ko je bil B. subtilis učinkovitejši proti po Gramu negativnim bakterijam.

Keywords

živinoreja;prehrana živali;krmen mešanice;mikrobiologija;probiotiki;Bacillus;antibiotiki;protimikrobna aktivnost;

Data

Language: Slovenian
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
Publisher: Biotehniška fakulteta
UDC: 636.084/.087
COBISS: 2412424 Link will open in a new window
ISSN: 1581-9175
Parent publication: Acta agriculturae Slovenica
Views: 1472
Downloads: 221
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Other data

Secondary language: English
Secondary title: Enumeration of probiotic microorganisms in feed mixtures
Secondary abstract: In animal nutrition a wide range of probiotic microorganisms belonging to lactic acid bacteria, yeasts and Bacillus genus are used. In this study we determined the number of microorganisms in different complete feed mixtures and premixtures, containing commercial probiotic supplements Bioplus 2b, Vebacor Biosaf. Bacillus subtilis and Bacillus licheniformis bacterial strains, which were isolated from product Bioplus 2b, were tested for antibiotic susceptibility, antimicrobial activity and ability to survive in simulated gastric and intestinal juice. We found that all tested products contained lower number of microorganisms than declared. The difference between declared and established value was the highest in products with Vebac and Biosaf. B. subtilis and B. licheniformis isolates were sensitive to all selected antibiotics except metronidazol. Survival test showed that simulated gastric or intestinal juices did not have considerable effect on spore survival, while vegetative cells of Bacillus strains were very sensitive to low pH and bile salts. B. licheniformis inhibited mostly Gram positive bacteria, while B. subtilis was more efficient against Gram negative bacteria.
Secondary keywords: animal husbandry;animal nutrition;feed mixtures;microbiology;probiotics;Bacillus;antibiotics;antimicrobial activity;
URN: URN:NBN:SI
Type (COBISS): Not categorized
Pages: str. 173-183
Volume: ǂLetn. ǂ92
Issue: ǂšt. ǂ2
Chronology: 2008
ID: 1734962