Tadej Tomanič (Author), Luka Rogelj (Author), Jošt Stergar (Author), Boštjan Markelc (Author), Tim Božič (Author), Simona Kranjc (Author), Gregor Serša (Author), Matija Milanič (Author)

Abstract

Understanding tumors and their microenvironment are essential for successful and accurate disease diagnosis. Tissue physiology and morphology are altered in tumors compared to healthy tissues, and there is a need to monitor tumors and their surrounding tissues, including blood vessels, non-invasively. This preliminary study utilizes a multimodal optical imaging system combining hyperspectral imaging (HSI) and threedimensional (3D) optical profilometry (OP) to capture hyperspectral images and surface shapes of subcutaneously grown murine tumor models. Hyperspectral images are corrected with 3D OP data and analyzed using the inverseadding doubling (IAD) method to extract tissue properties such as melanin volume fraction and oxygenation. Blood vessels are segmented using the B-COSFIRE algorithm from oxygenation maps. From 3D OP data, tumor volumes are calculated and compared to manual measurements using a vernier caliper. Results show that tumors can be distinguished from healthy tissue based on most extracted tissue parameters (p< 0.05). Furthermore, blood oxygenation is 50% higher within the blood vessels than in the surrounding tissue, and tumor volumes calculated using 3D OP agree within 26% with manual measurements using a vernier caliper. Results suggest that combining HSI and OP could provide relevant quantitative information about tumors and improve the disease diagnosis.

Keywords

medicinska fizika;hiperspektralno slikanje;difuzna refleksijska spektroskopija;ožilje;tumorji;medical physics;hyperspectral imaging;diffuse reflectance spectroscopy;blood vessels;tumors;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL FMF - Faculty of Mathematics and Physics
Publisher: Wiley
UDC: 616-073
COBISS: 117950211 Link will open in a new window
ISSN: 1864-0648
Views: 22
Downloads: 3
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Other data

Secondary language: Slovenian
Secondary keywords: medicinska fizika;hiperspektralno slikanje;difuzna refleksijska spektroskopija;ožilje;tumorji;
Type (COBISS): Article
Source comment: Nasl. z nasl. zaslona; Soavtorji: Luka Rogelj, Jost Stergar, Bostjan Markelc, Tim Bozic, Simona Kranjc Brezar, Gregor Sersa, Matija Milanic; Opis vira z dne 10. 8. 2022;
Pages: 28 str.
DOI: 10.1002/jbio.202200181
ID: 17842879