Targeted sequencing approach

comprehensive analysis of DNA methylation and gene expression across blood and brain regions in suicide victims

Katarina Kouter (Author), Tomaž Zupanc (Author), Alja Videtič (Author)

Abstract

Objectives Epigenetic mechanisms are involved in regulation of many pathologies, including suicidal behaviour. However, the factors through which epigenetics affect suicidal behaviour are not fully understood. Methods We analysed DNA methylation of eight neuropsychiatric genes (NR3C1, SLC6A4, HTR1A, TPH2, SKA2, MAOA, GABRA1, and NRIP3) in brain regions (hippocampus, insula, amygdala, Brodmann area 46) and blood of 25 male suicide victims and 28 male control subjects, using bisulfite next-generation sequencing. Results Comparing mean methylation values, notable changes were observed in NR3C1 (insula p-value =0.05), HTR1A (insula p-value <0.001, blood p-value =0.001), SKA2 (insula p-value =0.03, blood p-value =0.016), MAOA (blood p-value <0.001), GABRA1 (insula p-value =0.05, blood p-value =0.024) and NRIP3 (hippocampus p-value =0.001, insula p-value =0.002, amygdala p-value =0.014). Comparing methylation pattern between blood and brain, similarity was observed between blood and insula for HTR1A. Gene expression analysis in hippocampus revealed changes in expression of NR3C1 (p-value =0.049), SLC6A4 (p-value =0.017) and HTR1A (p-value =0.053). Conclusions Results provide an insight into the altered state of DNA methylation in suicidal behaviour. Epigenetic differences could therefore affect suicidal behaviour in both previously known and in novel neuropsychiatric candidate genes.

Keywords

suicidal behaviour;epigenetics;next-generation sequencing;

Data

Language:	English
Year of publishing:	2022
Typology:	1.01 - Original Scientific Article
Organization:	UL MF - Faculty of Medicine
UDC:	616.89
COBISS:	98996483
ISSN:	1814-1412
Views:	49
Downloads:	26
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	samomorilno vedenje;epigenetika;sekvenciranje naslednje generacije;
Type (COBISS):	Article
Pages:	str. 1-12
Issue:	ǂVol. ǂ9
Chronology:	2022
DOI:	10.1080/15622975.2022.2046291
ID:	17916555