magistrsko delo
Marcel Levstek (Author), Polona Žnidaršič Plazl (Mentor), Marjan Marinšek (Thesis defence commission member), Gabriela Kalčíková (Thesis defence commission member)

Abstract

Spore bakterije Bacillus subtilis so zaradi njihove odpornosti na različne okolijske dejavnike zanimive za mnoge biotehnološke uporabe. Razlog za njihovo odpornost je večplastna ovojnica, katere gradnik je tudi protein CotA, ki izraža aktivnost lakaz. Slednje so zmožne oksidacije raznovrstnih substratov, med katerimi je kot standard za določanje lakazne aktivnosti v rabi 2,2′-azino-bis(3-etilbenzotiazolin-6-sulfonska kislina) (ang. 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), ABTS). Uporaba spor B. subtilis kot biokatalizatorjev v mikropretočnih sistemih ima lahko številne prednosti pri razvoju miniaturiziranih biosenzorjev ali v pretočnih sistemih razgradnje fenolnih spojin. V sklopu magistrske naloge smo sprva preučili vpliv načina odmrzovanja spor na njihovo lakazno aktivnost, v nadaljevanju pa smo se osredotočili na razvoj matematičnega modela oksidacije ABTS v mikrobioreaktorju z imobiliziranimi sporami B. subtilis, s katerim je mogoče opisati in napovedati obnašanje procesa. Parametre encimske kinetike smo določili s prileganjem izpeljanega matematičnega modela na eksperimentalne podatke reakcije v pretočnem režimu, nato pa ovrednotili vpliv variacije procesnih spremenljivk na izkoristek procesa. Na koncu smo z modelom nestacionarnega stanja poskušali mehanistično opisati izgubo produktivnosti skozi čas obratovanja procesa, ki je posledica spiranja spor iz reaktorja.

Keywords

biotransformacije;Bacillus subtilis;biokatalizatorji;lakaze;matematično modeliranje;encimska kinetika;magistrska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [M. Levstek]
UDC: 66.098(043.2)
COBISS: 142802691 Link will open in a new window
Views: 56
Downloads: 10
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Other data

Secondary language: English
Secondary title: Mathematical modelling of a microbioreactor with immobilized Bacillus subtilis spores
Secondary abstract: Bacillus subtilis spores are a point of interest for many biotechnological applications, due to their resistance towards different environmental stresses. Their resistance comes from the multi-layered spore-coat, which also consists of the CotA protein that exhibits laccase activity. Laccases are capable of oxidizing different substrates, among which 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) is commonly used as a standard to determine their activity. The use of spores as biocatalysts in microflow systems has many advantages in the development of miniaturized biosensors or flow systems for degradation of phenolic substances. In the scope of this master’s thesis, the influence of spore thawing procedure on laccase activity was initially studied. Moreover, a mathematical model of ABTS oxidation in a microbioreactor with immobilized B. subtilis spores was developed, which can be used to describe and predict process behavior. Kinetic parameters were determined by fitting the derived mathematical model on experimental data obtained from a microflow system. With variation of process variables, their influence on the process yield was investigated. In the end, we tried to mechanistically describe the loss of productivity with a non-stationary model due to the process of spore flushing from the reactor.
Secondary keywords: Bacillus subtilis;biotransformations;mathematical modelling;microfluidics;Mikrofluidna tehnika;Mikroreaktorji;Univerzitetna in visokošolska dela;
Type (COBISS): Master's thesis/paper
Study programme: 1000376
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, smer Kemijsko inženirstvo
Pages: 68 str.
ID: 18003265