ATR-FTIR spectroscopy and chemometric complexity

unfolding the intra-skeleton variability

Irena Zupanič-Pajnič (Author), Tamara Leskovar (Author), Ivan Jerman (Author)

Abstract

Spectroscopy of skeletal tissues is increasingly used in various fields, including legal medicine, forensic science, and archaeology. As it is fast and technically and financially affordable yet accurate and widely applicable, spectroscopy is often used when investigating skeletal tissues. Despite its usefulness, the heterogeneity of skeletal tissues highly affects their chemical composition and complicates the interpretation of the spectroscopy results. Though the research on the use of spectroscopy and skeletal tissues from various contexts is growing, little is known regarding the differences in the chemometric indices caused by intra-skeleton variability. Objectives The objectives were a comparison of the chemometric indices between teeth and bones, between different bone classes, and between the skeletal elements within a bone class as well as an attempt to correlate the observed similarities or differences in chemometric indices to the functional or structural differences of skeletal elements. Materials and Methods Different skeletal elements of three individuals were analysed with attenuated total reflectance (ATR)–Fourier Transform Infrared (FTIR) spectroscopy and compared. Results Significant differences between the chemometric indices of the bones and teeth were observed and meaningfully correlated with their structural differences. Though more subtle and harder to understand, significant differences were also observed between and within the bone classes and were tentatively correlated with their structural and functional differences. Discussion The observed variability agrees with other studies that stress the importance of intra-skeleton variability, which should be acknowledged when using spectroscopy to investigate skeletal tissues.

Keywords

ATR-FTIR spektroskopija;znotrajskeletna variabilnost;kemometrija;ATR-FTIR spectroscopy;intra-skeletal variability;chemometrics;

Data

Language:	English
Year of publishing:	2022
Typology:	1.01 - Original Scientific Article
Organization:	UL MF - Faculty of Medicine
UDC:	61
COBISS:	123441923
ISSN:	1099-128X
Views:	23
Downloads:	3
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	ATR-FTIR spektroskopija;znotrajskeletna variabilnost;kemometrija;
Type (COBISS):	Article
Pages:	str. 1-15
Issue:	ǂVol. ǂ36
Chronology:	2022
DOI:	10.1002/cem.3448
ID:	18011589