Aleksandar Janev (Author), Taja Železnik (Author), Larisa Tratnjek (Author), Žiga Sardoč (Author), Hristina Obradović (Author), Slavko Mojsilović (Author), Milena Taskovska (Author), Tomaž Smrkolj (Author), Mateja Erdani-Kreft (Author)

Abstract

Despite being among the ten most common cancers with high recurrence rates worldwide, there have been no major breakthroughs in the standard treatment options for bladder cancer in recent years. The use of a human amniotic membrane (hAM) to treat cancer is one of the promising ideas that have emerged in recent years. This study aimed to investigate the anticancer activity of hAM homogenate on 2D and 3D cancer models. We evaluated the effects of hAM homogenates on the human muscle invasive bladder cancer urothelial (T24) cells, papillary cancer urothelial (RT4) cells and normal porcine urothelial (NPU) cells as well as on human mammary gland nontumorigenic (MCF10a) cells and low-metastatic breast cancer (MCF7) cells. After 24 h, we observed a gradual detachment of cancerous cells from the culture surface, while the hAM homogenate did not affect the normal cells. The most pronounced effect hAM homogenate had on bladder cancer cells; however, the potency of their detachment was dependent on the treatment protocol and the preparation of hAM homogenate. We demonstrated that hAM homogenate significantly decreased the adhesion, growth, and proliferation of human bladder invasive and papillary cancer urothelial cells and did not affect normal urothelial cells even in 7-day treatment. By using light and electron microscopy we showed that hAM homogenate disrupted the architecture of 2D and 3D bladder cancer models. The information provided by our study highlights the detrimental effect of hAM homogenate on bladder cancer cells and strengthens the idea of the potential clinical application of hAM for bladder cancer treatment.

Keywords

rak sečnega mehurja;urotelij;2D in 3D in vitro modeli;elektronski mikroskop;proliferacija;urologija;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL MF - Faculty of Medicine
UDC: 616.6
COBISS: 69118467 Link will open in a new window
ISSN: 2296-4185
Views: 19
Downloads: 4
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Other data

Secondary language: Slovenian
Secondary keywords: rak sečnega mehurja;urotelij;2D in 3D in vitro modeli;elektronski mikroskop;proliferacija;urologija;
Type (COBISS): Article
Pages: str. 1-19
Issue: ǂVol. ǂ9
Chronology: May 2021
DOI: 10.3389/fbioe.2021.690358
ID: 18187514
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