Samo Pirnat (Author), Mićo Božić (Author), Dorian Dolanc (Author), Anemari Horvat (Author), Petra Tavčar Verdev (Author), Nina Vardjan (Author), Alexei Verkhratsky (Author), Robert Zorec (Author), Matjaž Stenovec (Author)

Abstract

The plasticity of astrocytes is fundamental for their principal function, maintaining homeostasis of the central nervous system throughout life, and is associated with diverse exposomal challenges. Here, we used cultured astrocytes to investigate at subcellular level basic cell processes under controlled environmental conditions. We compared astroglial functional and signaling plasticity in standard serum-containing growth medium, a condition mimicking pathologic conditions, and in medium without serum, favoring the acquisition of arborized morphology. Using opto-/electrophysiologic techniques, we examined cell viability, expression of astroglial markers, vesicle dynamics, and cytosolic Ca2+ and cAMP signaling. The results revealed altered vesicle dynamics in arborized astrocytes that was associated with increased resting [Ca2 +]i and increased subcellular heterogeneity in [Ca2+]i, whereas [cAMP]i subcellular dynamics remained stable in both cultures, indicating that cAMP signaling is less prone to plastic remodeling than Ca2+ signaling, possibly also in in vivo contexts.

Keywords

astrocytes;vesicles;confocal microscopy;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL MF - Faculty of Medicine
UDC: 616-092
COBISS: 73561091 Link will open in a new window
ISSN: 0894-1491
Views: 32
Downloads: 4
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Other data

Secondary language: Slovenian
Secondary keywords: astrociti;vezikli;konfokalna mikroskopija;
Type (COBISS): Article
Pages: str. 2899-2916
Volume: ǂVol. ǂ69
Issue: ǂiss. ǂ12
Chronology: 2021
DOI: 10.1002/glia.24076
ID: 18220006