Efficient inactivation of MS-2 virus in water by hydrodynamic cavitation

Janez Kosel (Author), Ion Gutierrez-Aguirre (Author), Nejc Rački (Author), Tanja Dreo (Author), Maja Ravnikar (Author), Matevž Dular (Author)

Abstract

The aim of this study was to accurately quantify the impact of hydrodynamic cavitation on the infectivity of bacteriophage MS2, a norovirus surrogate, and to develop a small scale reactor for testing the effect of hydrodynamic cavitation on human enteric viruses, which cannot be easily prepared in large quantities. For this purpose, 3 mL scale and 1 L scale reactors were constructed and tested. Both devices were efficient in generating hydrodynamic cavitation and in reducing the infectivity of MS2 virus. Furthermore, they reached more than 4 logs reductions of viral infectivity, thus confirming the scalability of hydrodynamic cavitation for this particular application. As for the mechanism of page inactivation, we suspect that cavitation generated OH% radicals formed an advanced oxidation process, which could have damaged the host's recognition receptors located on the surface of the bacteriophage. Additional damage could arise from the high shear forces inside the cavity. Moreover, the effectiveness of the cavitation was higher for suspensions containing low initial viral titers that are in similar concentration to the ones found in real water samples. According to this, cavitation generators could prove to be a useful tool for treating virus-contaminated wastewaters in the future.

Keywords

MS2 bacteriophage;norovirus surrogate;hydrodynamic cavitation;Venturi type constriction;phage infectivity;

Data

Language:	English
Year of publishing:	2017
Typology:	1.01 - Original Scientific Article
Organization:	UL FS - Faculty of Mechanical Engineering
UDC:	578.2:532(045)
COBISS:	15600923
ISSN:	0043-1354
Views:	13
Downloads:	2
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	kavitacija;MS2 bakteriofag;venturijeva cev;virusi;infektivnost;
Type (COBISS):	Article
Embargo end date (OpenAIRE):	2019-08-08
Pages:	str. 465-471
Issue:	ǂVol. ǂ124
Chronology:	Nov. 2017
DOI:	10.1016/j.watres.2017.07.077
ID:	18275611