Ana Lisac (Author), Elfi Birsa (Author), Aleš Podgornik (Author)

Abstract

A system consisting of a connected mixed and tubular bioreactor was designed to study bacterial biofilm formation and the effect of its exposure to bacteriophages under different experimental conditions. The bacterial biofilm inside silicone tubular bioreactor was formed during the continuous pumping of bacterial cells at a constant physiological state for 2 h and subsequent washing with a buffer for 24 h. Monitoring bacterial and bacteriophage concentration along the tubular bioreactor was performed via a piercing method. The presence of biofilm and planktonic cells was demonstrated by combining the piercing method, measurement of planktonic cell concentration at the tubular bioreactor outlet, and optical microscopy. The planktonic cell formation rate was found to be 8.95 × 10$^{−3}$ h$^{−1}$ and increased approximately four-fold (4×) after biofilm exposure to an LB medium. Exposure of bacterial biofilm to bacteriophages in the LB medium resulted in a rapid decrease of biofilm and planktonic cell concentration, to below the detection limit within < 2 h. When bacteriophages were supplied in the buffer, only a moderate decrease in the concentration of both bacterial cell types was observed. After biofilm washing with buffer to remove unadsorbed bacteriophages, its exposure to the LB medium (without bacteriophages) resulted in a rapid decrease in bacterial concentration: again below the detection limit in < 2 h.

Keywords

bakterijski biofilm;kontinuirni cevni bioreaktor;bakteriofagna interakcija;E. coli;bakteriofag T4;microbial biofilm;continuous tubular bioreactor;bacteriophage interaction;bacteriophage T4;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
UDC: 602.3:578.347
COBISS: 110219011 Link will open in a new window
ISSN: 1751-7915
Views: 21
Downloads: 5
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Other data

Secondary language: Slovenian
Secondary keywords: bakterijski biofilm;kontinuirni cevni bioreaktor;bakteriofagna interakcija;E. coli;bakteriofag T4;
Type (COBISS): Article
Pages: str. 2450-2463
Volume: ǂVol. ǂ15
Issue: ǂiss. ǂ9
Chronology: Sep. 2022
DOI: 10.1111/1751-7915.14079
ID: 18670121