Anna Janosity (Author), József Baranyi (Author), Botond Bendegúz Surányi (Author), Sonja Smole Možina (Author), Andrea Taczman-Brückner (Author), Gabriella Kiskó (Author), Anja Klančnik (Author)

Abstract

Our aim was to find the optimal efflux inhibitor concentration of a natural component, carvacrol, as a function of the physiological state of Escherichia coli. Using fluorescence-based measurements with two strains of E. coli, the effect of carvacrol was assessed at 17 sub-inhibitory concentrations, at which the bacterial efflux mechanism was compromised. The efficacy of carvacrol, as an efflux inhibitor, was compared to synthetic inhibitors and we found carvacrol the most efficient one. We considered the accumulation of Ethidium Bromide (EtBr) as a proxy for drugs spreading in the cell, thus measuring the efflux activity indirectly. The change in membrane integrity caused by the exposure to carvacrol was monitored using the LIVE/DEAD BacLight Bacterial Viability kit. To find the optimal inhibitory concentration of carvacrol, we used predictive microbiology methods. This optimum varied with the bacterial physiological state, as non-growing cultures were less susceptible to the effect of carvacrol than growing cultures were. Moreover, we point out, for the first time, that the efflux-mediated resistance of untreated cultures was also stronger in the non-growing than in the growing phase at population level.

Keywords

protimikrobne snovi;karvakrol;izlivne črpalke;napovedna mikrobiologija;efflux inhibitor;carvacrol;physiological-state-dependent resistance;fluorescence-based assays;predictive modeling;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 579.2:547.9
COBISS: 139735299 Link will open in a new window
ISSN: 1664-302X
Views: 182
Downloads: 40
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Other data

Secondary language: Slovenian
Secondary keywords: protimikrobne snovi;karvakrol;izlivne črpalke;napovedna mikrobiologija;
Type (COBISS): Article
Pages: str. [1-10]
Volume: ǂVol. ǂ14
Issue: [article no.] 1073798
Chronology: 2023
DOI: 10.3389/fmicb.2023.1073798
ID: 18873815